Adult hepatocytes are generated by self-duplication rather than stem cell differentiation

Kilangsungla Yanger; David Knigin; Yiwei Zong; Lara Maggs; Guoqiang Gu; Haruhiko Akiyama; Eli Pikarsky; Ben Z. Stanger

doi:10.1016/j.stem.2014.06.003

Adult hepatocytes are generated by self-duplication rather than stem cell differentiation

Kilangsungla Yanger, David Knigin, Yiwei Zong, Lara Maggs, Guoqiang Gu, Haruhiko Akiyama, Eli Pikarsky, Ben Z. Stanger^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

357 Scopus citations

Abstract

The liver is thought to utilize facultative stem cells, also known as "oval cells" or "atypical ductal cells" (ADCs), for regeneration following various types of injury. However, this notion has been based largely on in vitro studies and transplantation models; where lineage tracing has been used, results have been conflicting and effect sizes have been small. Here, we used genetic and nucleoside analog-based tools to mark and track the origin and contribution of various cell populations to liver regeneration in vivo following several ADC-inducing insults. We report that, contrary to prevailing stem-cell-based models of regeneration, virtually all new hepatocytes come from preexisting hepatocytes.

Original language	English
Pages (from-to)	340-349
Number of pages	10
Journal	Cell Stem Cell
Volume	15
Issue number	3
DOIs	https://doi.org/10.1016/j.stem.2014.06.003
State	Published - 2014
Externally published	Yes

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© 2014 Elsevier Inc.

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AU - Knigin, David

AU - Zong, Yiwei

AU - Maggs, Lara

AU - Gu, Guoqiang

AU - Akiyama, Haruhiko

AU - Pikarsky, Eli

AU - Stanger, Ben Z.

PY - 2014

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Adult hepatocytes are generated by self-duplication rather than stem cell differentiation

Abstract

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