TY - JOUR
T1 - Advanced backcross QTL analysis of a Lycopersicon esculentum x Lycopersicon parviflorum cross
AU - Fulton, T. M.
AU - Grandillo, S.
AU - Beck-Bunn, T.
AU - Fridman, E.
AU - Frampton, A.
AU - Lopez, J.
AU - Petiard, V.
AU - Uhlig, J.
AU - Zamir, D.
AU - Tanksley, S. D.
PY - 2000
Y1 - 2000
N2 - Lycopersicon parviflorum is a sexually compatible, wild tomato species which has been largely unutilized in tomato breeding. The Advanced Backcross QTL (AB-QTL) strategy was used to explore this genome for QTLs affecting traits of agronomic importance in an interspecific cross between a tomato elite processing inbred, Lycopersicon esculentum E6203, and the wild species L. parviflorum (LA2133). A total of 170 BC2 plants were genotyped by means of 133 genetic markers (131 RFLPs; one PCR-based marker, 1-2, and one morphological marker, u, uniform ripening). Approximately 170 BC3 families were grown in replicated field trials, in California, Spain and Israel, and were scored for 30 horticultural traits. Significant putative QTLs were identified for all traits, for a total of 199 QTLs, ranging from 1 to 19 QTLs detected for each trait. For 19 (70%) traits (excluding traits for which effects of either direction are not necessarily favourable or unfavourable) at least one QTL was identified for which the L. parviflorum allele was associated with an agronomically favourable effect, despite the overall inferior phenotype of the wild species.
AB - Lycopersicon parviflorum is a sexually compatible, wild tomato species which has been largely unutilized in tomato breeding. The Advanced Backcross QTL (AB-QTL) strategy was used to explore this genome for QTLs affecting traits of agronomic importance in an interspecific cross between a tomato elite processing inbred, Lycopersicon esculentum E6203, and the wild species L. parviflorum (LA2133). A total of 170 BC2 plants were genotyped by means of 133 genetic markers (131 RFLPs; one PCR-based marker, 1-2, and one morphological marker, u, uniform ripening). Approximately 170 BC3 families were grown in replicated field trials, in California, Spain and Israel, and were scored for 30 horticultural traits. Significant putative QTLs were identified for all traits, for a total of 199 QTLs, ranging from 1 to 19 QTLs detected for each trait. For 19 (70%) traits (excluding traits for which effects of either direction are not necessarily favourable or unfavourable) at least one QTL was identified for which the L. parviflorum allele was associated with an agronomically favourable effect, despite the overall inferior phenotype of the wild species.
KW - Germplasm utilization
KW - Introgression
KW - L. parviflorum
KW - Molecular breeding
KW - Quantitative trait loci
KW - Tomato
UR - http://www.scopus.com/inward/record.url?scp=0034127743&partnerID=8YFLogxK
U2 - 10.1007/s001220051384
DO - 10.1007/s001220051384
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AN - SCOPUS:0034127743
SN - 0040-5752
VL - 100
SP - 1025
EP - 1042
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
IS - 7
ER -