Adventitious shoot formation from carnation stem segments: A comparison of different culture procedures

Abed A. Watad*, Asaf Ahroni, Amir Zuker, Hadas Shejtman, Ada Nissim, Alexander Vainstein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Adventitious shoot regeneration from stem explants of carnation (Dianthus caryophyllus L., cultivar 'White Sim') was studied using three different culture procedures: agar-gelled medium, liquid shaken medium, and an interfacial membrane raft floating on liquid medium. In all culture procedures, explants derived from the first upper internode exhibited higher adventitious shoot formation than those from the second internode. On agar- gelled medium, the number of regenerated shoots per explant from the first internode increased with increasing thidiazuron (TDZ) concentration (up to 4 mg 1-1) in the presence of 1 mg 1-1 naphthaleneacetic acid. In the liquid shaken culture, the number of regenerated shoots peaked at 1 mg TDZ 1-1. Maximum shoot regeneration on an interfacial membrane raft was obtained in the presence of 0.25 mg TDZ 1-1. A comparison of the overall efficiencies of shoot regeneration for each culture procedure showed that maximum shoot regeneration on the raft was twice and three times that obtained with agar- gelled and liquid shaken media, respectively.

Original languageAmerican English
Pages (from-to)313-320
Number of pages8
JournalScientia Horticulturae
Volume65
Issue number4
DOIs
StatePublished - Aug 1996

Bibliographical note

Funding Information:
This researchw as supportedb y Grant No. US-2233-92f rom BARD, the United States-IsraeBl inationalA griculturalR esearcha nd DevelopmenFtu nd, by the Israeli Ministry of Agriculturea nd by the Israeli Flower GrowersA ssociationT. he authors wish to thankD r. Y. Ben-Tal for his critical commentso n the manuscripat nd Shemi Ltd. (ShdemaI,s rael)f or providingp lantm aterial.

Keywords

  • Agar gelled medium
  • Interfacial membrane raft
  • Liquid medium
  • Shoot regeneration

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