TY - JOUR
T1 - Affinity label for β adrenergic receptor in turkey erythrocytes
AU - Atlas, D.
AU - Steer, M. L.
AU - Levitzki, A.
PY - 1976
Y1 - 1976
N2 - The compound N [2 hydroxy 3 (1 naphthoxy) propyl] N' bromoacetylethylenediamine (NHNP NBE) was found to label covalently the β adrenergic receptor in turkey erythrocytes. The compound inhibits irreversibly l epinephrine dependent adenylate cyclase activity [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] in the whole turkey erythrocyte as well as in the erythrocyte membranes possessing the β receptor. The affinity label blocks, also irreversibly, the specific [3H]propranolol binding, whereas other bromoacetyl compounds tested have no effect on binding, even at high concentrations, which cause enzyme inactivation. l Epinephrine and propranolol offer protection against the affinity label in whole turkey erythrocytes as well as in membranes prepared from these cells. The potential usefulness of an irreversible β antagonist is discussed.
AB - The compound N [2 hydroxy 3 (1 naphthoxy) propyl] N' bromoacetylethylenediamine (NHNP NBE) was found to label covalently the β adrenergic receptor in turkey erythrocytes. The compound inhibits irreversibly l epinephrine dependent adenylate cyclase activity [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] in the whole turkey erythrocyte as well as in the erythrocyte membranes possessing the β receptor. The affinity label blocks, also irreversibly, the specific [3H]propranolol binding, whereas other bromoacetyl compounds tested have no effect on binding, even at high concentrations, which cause enzyme inactivation. l Epinephrine and propranolol offer protection against the affinity label in whole turkey erythrocytes as well as in membranes prepared from these cells. The potential usefulness of an irreversible β antagonist is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0017116459&partnerID=8YFLogxK
U2 - 10.1073/pnas.73.6.1921
DO - 10.1073/pnas.73.6.1921
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C2 - 180528
AN - SCOPUS:0017116459
SN - 0027-8424
VL - 73
SP - 1921
EP - 1925
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 6
ER -
