Abstract
The compound N [2 hydroxy 3 (1 naphthoxy) propyl] N' bromoacetylethylenediamine (NHNP NBE) was found to label covalently the β adrenergic receptor in turkey erythrocytes. The compound inhibits irreversibly l epinephrine dependent adenylate cyclase activity [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] in the whole turkey erythrocyte as well as in the erythrocyte membranes possessing the β receptor. The affinity label blocks, also irreversibly, the specific [3H]propranolol binding, whereas other bromoacetyl compounds tested have no effect on binding, even at high concentrations, which cause enzyme inactivation. l Epinephrine and propranolol offer protection against the affinity label in whole turkey erythrocytes as well as in membranes prepared from these cells. The potential usefulness of an irreversible β antagonist is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 1921-1925 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 73 |
| Issue number | 6 |
| DOIs | |
| State | Published - 1976 |
Fingerprint
Dive into the research topics of 'Affinity label for β adrenergic receptor in turkey erythrocytes'. Together they form a unique fingerprint.Cite this
- APA
- Author
- BIBTEX
- Harvard
- Standard
- RIS
- Vancouver