Age trends in estradiol and estrone levels measured using liquid chromatography tandem mass spectrometry in community-dwelling men of the framingham heart study

Guneet Kaur Jasuja; Thomas G. Travison; Maithili Davda; Joanne M. Murabito; Shehzad Basaria; Anqi Zhang; Mark M. Kushnir; Alan L. Rockwood; Wayne Meikle; Michael J. Pencina; Andrea Coviello; Adam J. Rose; Ralph D'Agostino; Ramachandran S. Vasan; Shalender Bhasin

doi:10.1093/gerona/gls216

Age trends in estradiol and estrone levels measured using liquid chromatography tandem mass spectrometry in community-dwelling men of the framingham heart study

Guneet Kaur Jasuja, Thomas G. Travison, Maithili Davda, Joanne M. Murabito, Shehzad Basaria, Anqi Zhang, Mark M. Kushnir, Alan L. Rockwood, Wayne Meikle, Michael J. Pencina, Andrea Coviello, Adam J. Rose, Ralph D'Agostino, Ramachandran S. Vasan, Shalender Bhasin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

68 Scopus citations

Abstract

Background.Age trends in estradiol and estrone levels in men and how lifestyle factors, comorbid conditions, testosterone, and sex hormone-binding globulin affect these age trends remain poorly understood, and were examined in men of the Framingham Heart Study.Methods.Estrone and estradiol concentrations were measured in morning fasting samples using liquid chromatography tandem mass spectrometry in men of Framingham Offspring Generation. Free estradiol was calculated using a law of mass action equation.Results.There were 1,461 eligible men (mean age [±SD] 61.1±9.5 years and body mass index [BMI] 28.8±4.5kg/m). Total estradiol and estrone were positively associated with age, but free estradiol was negatively associated with age. Age-related increase in total estrone was greater than that in total estradiol. Estrone was positively associated with smoking, BMI, and testosterone, and total and free estradiol with diabetes, BMI, testosterone, and comorbid conditions; additionally, free estradiol was associated negatively with smoking. Collectively, age, BMI, testosterone, and other health and behavioral factors explained only 18% of variance in estradiol, and 9% of variance in estrone levels. Men in the highest quintile of estrone levels had significantly higher age and BMI, and a higher prevalence of smoking, diabetes, and cardiovascular disease than others, whereas those in the highest quintile of estradiol had higher BMI than others.Conclusions.Total estrone and estradiol levels in men, measured using liquid chromatography tandem mass spectrometry, revealed significant age-related increases that were only partially accounted for by cross-sectional differences in BMI, diabetes status, and other comorbidities and health behaviors. Longitudinal studies are needed to confirm these findings.

Original language	English
Pages (from-to)	733-740
Number of pages	8
Journal	Journals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume	68
Issue number	6
DOIs	https://doi.org/10.1093/gerona/gls216
State	Published - Jun 2013
Externally published	Yes

Bibliographical note

Funding Information:
Funding This project was supported by National Institute on Aging grant 1RO1AG31206 to S.B. and R.S.V. Additional support was provided by the Boston Claude D. Pepper Older Americans Independence Center (5P30AG031679) and by a grant from the CDC Foundation. The Framingham Heart Study is supported by the National Heart, Lung and Blood Institute’s contract N01-HC-25195.

Keywords

Age trends
Age-related changes in estrone and estradiol
Determinants of estrogen levels in men
Estrogen levels in men
LC-MS/MS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/gerona/gls216

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Jasuja, G. K., Travison, T. G., Davda, M., Murabito, J. M., Basaria, S., Zhang, A., Kushnir, M. M., Rockwood, A. L., Meikle, W., Pencina, M. J., Coviello, A., Rose, A. J., D'Agostino, R., Vasan, R. S., & Bhasin, S. (2013). Age trends in estradiol and estrone levels measured using liquid chromatography tandem mass spectrometry in community-dwelling men of the framingham heart study. Journals of Gerontology - Series A Biological Sciences and Medical Sciences, 68(6), 733-740. https://doi.org/10.1093/gerona/gls216

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title = "Age trends in estradiol and estrone levels measured using liquid chromatography tandem mass spectrometry in community-dwelling men of the framingham heart study",

abstract = "Background.Age trends in estradiol and estrone levels in men and how lifestyle factors, comorbid conditions, testosterone, and sex hormone-binding globulin affect these age trends remain poorly understood, and were examined in men of the Framingham Heart Study.Methods.Estrone and estradiol concentrations were measured in morning fasting samples using liquid chromatography tandem mass spectrometry in men of Framingham Offspring Generation. Free estradiol was calculated using a law of mass action equation.Results.There were 1,461 eligible men (mean age [±SD] 61.1±9.5 years and body mass index [BMI] 28.8±4.5kg/m). Total estradiol and estrone were positively associated with age, but free estradiol was negatively associated with age. Age-related increase in total estrone was greater than that in total estradiol. Estrone was positively associated with smoking, BMI, and testosterone, and total and free estradiol with diabetes, BMI, testosterone, and comorbid conditions; additionally, free estradiol was associated negatively with smoking. Collectively, age, BMI, testosterone, and other health and behavioral factors explained only 18% of variance in estradiol, and 9% of variance in estrone levels. Men in the highest quintile of estrone levels had significantly higher age and BMI, and a higher prevalence of smoking, diabetes, and cardiovascular disease than others, whereas those in the highest quintile of estradiol had higher BMI than others.Conclusions.Total estrone and estradiol levels in men, measured using liquid chromatography tandem mass spectrometry, revealed significant age-related increases that were only partially accounted for by cross-sectional differences in BMI, diabetes status, and other comorbidities and health behaviors. Longitudinal studies are needed to confirm these findings.",

keywords = "Age trends, Age-related changes in estrone and estradiol, Determinants of estrogen levels in men, Estrogen levels in men, LC-MS/MS",

author = "Jasuja, {Guneet Kaur} and Travison, {Thomas G.} and Maithili Davda and Murabito, {Joanne M.} and Shehzad Basaria and Anqi Zhang and Kushnir, {Mark M.} and Rockwood, {Alan L.} and Wayne Meikle and Pencina, {Michael J.} and Andrea Coviello and Rose, {Adam J.} and Ralph D'Agostino and Vasan, {Ramachandran S.} and Shalender Bhasin",

note = "Funding Information: Funding This project was supported by National Institute on Aging grant 1RO1AG31206 to S.B. and R.S.V. Additional support was provided by the Boston Claude D. Pepper Older Americans Independence Center (5P30AG031679) and by a grant from the CDC Foundation. The Framingham Heart Study is supported by the National Heart, Lung and Blood Institute{\textquoteright}s contract N01-HC-25195.",

year = "2013",

month = jun,

doi = "10.1093/gerona/gls216",

language = "אנגלית",

volume = "68",

pages = "733--740",

journal = "Journals of Gerontology - Series A Biological Sciences and Medical Sciences",

issn = "1079-5006",

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number = "6",

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Jasuja, GK, Travison, TG, Davda, M, Murabito, JM, Basaria, S, Zhang, A, Kushnir, MM, Rockwood, AL, Meikle, W, Pencina, MJ, Coviello, A, Rose, AJ, D'Agostino, R, Vasan, RS & Bhasin, S 2013, 'Age trends in estradiol and estrone levels measured using liquid chromatography tandem mass spectrometry in community-dwelling men of the framingham heart study', Journals of Gerontology - Series A Biological Sciences and Medical Sciences, vol. 68, no. 6, pp. 733-740. https://doi.org/10.1093/gerona/gls216

Age trends in estradiol and estrone levels measured using liquid chromatography tandem mass spectrometry in community-dwelling men of the framingham heart study. / Jasuja, Guneet Kaur; Travison, Thomas G.; Davda, Maithili et al.
In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 68, No. 6, 06.2013, p. 733-740.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Age trends in estradiol and estrone levels measured using liquid chromatography tandem mass spectrometry in community-dwelling men of the framingham heart study

AU - Jasuja, Guneet Kaur

AU - Travison, Thomas G.

AU - Davda, Maithili

AU - Murabito, Joanne M.

AU - Basaria, Shehzad

AU - Zhang, Anqi

AU - Kushnir, Mark M.

AU - Rockwood, Alan L.

AU - Meikle, Wayne

AU - Pencina, Michael J.

AU - Coviello, Andrea

AU - Rose, Adam J.

AU - D'Agostino, Ralph

AU - Vasan, Ramachandran S.

AU - Bhasin, Shalender

N1 - Funding Information: Funding This project was supported by National Institute on Aging grant 1RO1AG31206 to S.B. and R.S.V. Additional support was provided by the Boston Claude D. Pepper Older Americans Independence Center (5P30AG031679) and by a grant from the CDC Foundation. The Framingham Heart Study is supported by the National Heart, Lung and Blood Institute’s contract N01-HC-25195.

PY - 2013/6

Y1 - 2013/6

N2 - Background.Age trends in estradiol and estrone levels in men and how lifestyle factors, comorbid conditions, testosterone, and sex hormone-binding globulin affect these age trends remain poorly understood, and were examined in men of the Framingham Heart Study.Methods.Estrone and estradiol concentrations were measured in morning fasting samples using liquid chromatography tandem mass spectrometry in men of Framingham Offspring Generation. Free estradiol was calculated using a law of mass action equation.Results.There were 1,461 eligible men (mean age [±SD] 61.1±9.5 years and body mass index [BMI] 28.8±4.5kg/m). Total estradiol and estrone were positively associated with age, but free estradiol was negatively associated with age. Age-related increase in total estrone was greater than that in total estradiol. Estrone was positively associated with smoking, BMI, and testosterone, and total and free estradiol with diabetes, BMI, testosterone, and comorbid conditions; additionally, free estradiol was associated negatively with smoking. Collectively, age, BMI, testosterone, and other health and behavioral factors explained only 18% of variance in estradiol, and 9% of variance in estrone levels. Men in the highest quintile of estrone levels had significantly higher age and BMI, and a higher prevalence of smoking, diabetes, and cardiovascular disease than others, whereas those in the highest quintile of estradiol had higher BMI than others.Conclusions.Total estrone and estradiol levels in men, measured using liquid chromatography tandem mass spectrometry, revealed significant age-related increases that were only partially accounted for by cross-sectional differences in BMI, diabetes status, and other comorbidities and health behaviors. Longitudinal studies are needed to confirm these findings.

AB - Background.Age trends in estradiol and estrone levels in men and how lifestyle factors, comorbid conditions, testosterone, and sex hormone-binding globulin affect these age trends remain poorly understood, and were examined in men of the Framingham Heart Study.Methods.Estrone and estradiol concentrations were measured in morning fasting samples using liquid chromatography tandem mass spectrometry in men of Framingham Offspring Generation. Free estradiol was calculated using a law of mass action equation.Results.There were 1,461 eligible men (mean age [±SD] 61.1±9.5 years and body mass index [BMI] 28.8±4.5kg/m). Total estradiol and estrone were positively associated with age, but free estradiol was negatively associated with age. Age-related increase in total estrone was greater than that in total estradiol. Estrone was positively associated with smoking, BMI, and testosterone, and total and free estradiol with diabetes, BMI, testosterone, and comorbid conditions; additionally, free estradiol was associated negatively with smoking. Collectively, age, BMI, testosterone, and other health and behavioral factors explained only 18% of variance in estradiol, and 9% of variance in estrone levels. Men in the highest quintile of estrone levels had significantly higher age and BMI, and a higher prevalence of smoking, diabetes, and cardiovascular disease than others, whereas those in the highest quintile of estradiol had higher BMI than others.Conclusions.Total estrone and estradiol levels in men, measured using liquid chromatography tandem mass spectrometry, revealed significant age-related increases that were only partially accounted for by cross-sectional differences in BMI, diabetes status, and other comorbidities and health behaviors. Longitudinal studies are needed to confirm these findings.

KW - Age trends

KW - Age-related changes in estrone and estradiol

KW - Determinants of estrogen levels in men

KW - Estrogen levels in men

KW - LC-MS/MS

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JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences

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Age trends in estradiol and estrone levels measured using liquid chromatography tandem mass spectrometry in community-dwelling men of the framingham heart study

Abstract

Bibliographical note

Keywords

UN SDGs

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