Age trends in estradiol and estrone levels measured using liquid chromatography tandem mass spectrometry in community-dwelling men of the framingham heart study

Guneet Kaur Jasuja, Thomas G. Travison, Maithili Davda, Joanne M. Murabito, Shehzad Basaria, Anqi Zhang, Mark M. Kushnir, Alan L. Rockwood, Wayne Meikle, Michael J. Pencina, Andrea Coviello, Adam J. Rose, Ralph D'Agostino, Ramachandran S. Vasan, Shalender Bhasin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


Background.Age trends in estradiol and estrone levels in men and how lifestyle factors, comorbid conditions, testosterone, and sex hormone-binding globulin affect these age trends remain poorly understood, and were examined in men of the Framingham Heart Study.Methods.Estrone and estradiol concentrations were measured in morning fasting samples using liquid chromatography tandem mass spectrometry in men of Framingham Offspring Generation. Free estradiol was calculated using a law of mass action equation.Results.There were 1,461 eligible men (mean age [±SD] 61.1±9.5 years and body mass index [BMI] 28.8±4.5kg/m). Total estradiol and estrone were positively associated with age, but free estradiol was negatively associated with age. Age-related increase in total estrone was greater than that in total estradiol. Estrone was positively associated with smoking, BMI, and testosterone, and total and free estradiol with diabetes, BMI, testosterone, and comorbid conditions; additionally, free estradiol was associated negatively with smoking. Collectively, age, BMI, testosterone, and other health and behavioral factors explained only 18% of variance in estradiol, and 9% of variance in estrone levels. Men in the highest quintile of estrone levels had significantly higher age and BMI, and a higher prevalence of smoking, diabetes, and cardiovascular disease than others, whereas those in the highest quintile of estradiol had higher BMI than others.Conclusions.Total estrone and estradiol levels in men, measured using liquid chromatography tandem mass spectrometry, revealed significant age-related increases that were only partially accounted for by cross-sectional differences in BMI, diabetes status, and other comorbidities and health behaviors. Longitudinal studies are needed to confirm these findings.

Original languageAmerican English
Pages (from-to)733-740
Number of pages8
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Issue number6
StatePublished - Jun 2013
Externally publishedYes

Bibliographical note

Funding Information:
Funding This project was supported by National Institute on Aging grant 1RO1AG31206 to S.B. and R.S.V. Additional support was provided by the Boston Claude D. Pepper Older Americans Independence Center (5P30AG031679) and by a grant from the CDC Foundation. The Framingham Heart Study is supported by the National Heart, Lung and Blood Institute’s contract N01-HC-25195.


  • Age trends
  • Age-related changes in estrone and estradiol
  • Determinants of estrogen levels in men
  • Estrogen levels in men
  • LC-MS/MS


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