TY - JOUR
T1 - Aged keratinocyte phenotyping
T2 - Morphology, biochemical markers and effects of Dead Sea minerals
AU - Soroka, Yoram
AU - Ma'or, Zeev
AU - Leshem, Yael
AU - Verochovsky, Lilian
AU - Neuman, Rami
AU - Brégégère, François Menahem
AU - Milner, Yoram
PY - 2008/10
Y1 - 2008/10
N2 - The aging process and its characterization in keratinocytes have not been studied in depth until now. We have assessed the cellular and molecular characteristics of aged epidermal keratinocytes in monolayer cultures and in skin by measuring their morphological, fluorometric and biochemical properties. Light and electron microscopy, as well as flow cytometry, revealed increase in cell size, changes in cell shape, alterations in mitochondrial structure and cytoplasmic content with aging. We showed that the expression of 16 biochemical markers was altered in aged cultured cells and in tissues, including caspases 1 and 3 and β-galactosidase activities, immunoreactivities of p16, Ki67, 20S proteasome and effectors of the Fas-dependent apoptotic pathway. Aged cells diversity, and individual variability of aging markers, call for a multifunctional assessment of the aging phenomenon, and of its modulation by drugs. As a test case, we have measured the effects of Dead Sea minerals on keratinocyte cultures and human skin, and found that they stimulate proliferation and mitochondrial activity, decrease the expression of some aging markers, and limit apoptotic damage after UVB irradiation.
AB - The aging process and its characterization in keratinocytes have not been studied in depth until now. We have assessed the cellular and molecular characteristics of aged epidermal keratinocytes in monolayer cultures and in skin by measuring their morphological, fluorometric and biochemical properties. Light and electron microscopy, as well as flow cytometry, revealed increase in cell size, changes in cell shape, alterations in mitochondrial structure and cytoplasmic content with aging. We showed that the expression of 16 biochemical markers was altered in aged cultured cells and in tissues, including caspases 1 and 3 and β-galactosidase activities, immunoreactivities of p16, Ki67, 20S proteasome and effectors of the Fas-dependent apoptotic pathway. Aged cells diversity, and individual variability of aging markers, call for a multifunctional assessment of the aging phenomenon, and of its modulation by drugs. As a test case, we have measured the effects of Dead Sea minerals on keratinocyte cultures and human skin, and found that they stimulate proliferation and mitochondrial activity, decrease the expression of some aging markers, and limit apoptotic damage after UVB irradiation.
KW - Biomarker profiling
KW - Dead Sea minerals
KW - Keratinocyte senescence
KW - Photodamage
KW - Skin aging markers
UR - https://www.scopus.com/pages/publications/53049096667
U2 - 10.1016/j.exger.2008.08.003
DO - 10.1016/j.exger.2008.08.003
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C2 - 18761079
AN - SCOPUS:53049096667
SN - 0531-5565
VL - 43
SP - 947
EP - 957
JO - Experimental Gerontology
JF - Experimental Gerontology
IS - 10
ER -