Agents that inhibit Rho, Rac, and Cdc42 do not block formation of actin pedestals in HeLa cells infected with enteropathogenic Escherichia coli.

G. Ben-Ami*, V. Ozeri, E. Hanski, F. Hofmann, K. Aktories, K. M. Hahn, G. M. Bokoch, I. Rosenshine

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Enteropathogenic Escherichia coli (EPEC) induces formation of actin pedestals in infected host cells. Agents that inhibit the activity of Rho, Rac, and Cdc42, including Clostridium difficile toxin B (ToxB), compactin, and dominant negative Rho, Rac, and Cdc42, did not inhibit formation of actin pedestals. In contrast, treatment of HeLa cells with ToxB inhibited EPEC invasion. Thus, Rho, Rac, and Cdc42 are not required for assembly of actin pedestals; however, they may be involved in EPEC uptake by HeLa cells.

Original languageEnglish
Pages (from-to)1755-1758
Number of pages4
JournalInfection and Immunity
Volume66
Issue number4
DOIs
StatePublished - Apr 1998

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