Agrobacterium-mediated transformation of gypsophila (Gypsophila paniculata L.)

Michal Moyal Ben Zvi, Amir Zuker, Marianna Ovadis, Elena Shklarman, Hagit Ben-Meir, Shamir Zenvirt, Alexander Vainstein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

As a major contributor to the flower market, Gypsophila paniculata is an important target for the breeding of new varieties. However, gypsophila breeding is strongly hampered by the sterility of this species' genotypes and the lack of a genetic-transformation procedure for this genus. Here we describe the establishment of a transformation procedure for gypsophila (Gypsophila paniculata L.) based on Agrobacterium inoculation of highly regenerative stem segments. The transformation procedure employs stem explants derived from GA3-pretreated mother plants and a two-step selection scheme. The GA3 treatment was crucial for obtaining high gene-transfer frequencies (75-90% GUS-expressing explants out of total inoculated explants), as shown using three different gypsophila varieties. An overall transformation efficiency of five GUS-expressing shoots per 100 stem explants was demonstrated for cv. Arbel. The applicability of the transformation system to gypsophila was further reinforced by the generation of transgenic plants expressing Agrobacterium rhizogenes rolC driven by a CaMV 35S promoter. Transgenic gypsophila plantlets exhibited extensive rooting and branching, traits that could be beneficial to the ornamental industry.

Original languageAmerican English
Pages (from-to)543-553
Number of pages11
JournalMolecular Breeding
Volume22
Issue number4
DOIs
StatePublished - Nov 2008

Bibliographical note

Funding Information:
Acknowledgements The authors wish to thank Danziger ‘‘DAN’’ Flower Farm (Moshav Mishmar Hashiva, Israel) for providing the plant material. This research was supported by grant number 256-0715-06 from the Chief Scientist of the Israel Ministry of Agriculture and Rural Development.

Keywords

  • Genetic transformation
  • Gypsophila
  • Ornamentals
  • rolC

Fingerprint

Dive into the research topics of 'Agrobacterium-mediated transformation of gypsophila (Gypsophila paniculata L.)'. Together they form a unique fingerprint.

Cite this