Alkane hydroxylation by cytochrome P450: Is kinetic isotope effect a reliable probe of transition state structure?

François Ogliaro, Michael Filatov, Sason Shaik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Computed kinetic isotope effect (KIE) values for methane hydroxylation by Cytochrome P450, in both high- and low-spin states, compare well with KIE's for model hydrogen abstraction reactions, thereby demonstrating the utility of KIE as a mechanistic probe and supporting a two-state-rebound mechanism.

Original languageEnglish
Pages (from-to)2455-2458
Number of pages4
JournalEuropean Journal of Inorganic Chemistry
Issue number12
DOIs
StatePublished - Dec 2000

Keywords

  • Cytochrome P450
  • Density functional calculations
  • Hydroxylation
  • Isotope effects
  • Metalloenzymes

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