Allele-specific expression patterns of interleukin-2 end Pax-5 revealed by a sensitive single-cell RT-PCR analysis

Kristina L. Rhoades, Nandita Singh, Itamar Simon, Barbara Glidden, Howard Cedar, Andrew Chess*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

Autosomal genes that are subject to random allelic inactivation (RAI), like imprinted genes [1] end genes subject to X-inactivation [2], require mechanisms that dictate the differential transcriptional regulation of two sequence-identical alleles. RAI genes include olfactory receptor genes [3], and the various genes encoding antigen receptors on lymphocytes (immunoglobulin genes, T cell receptor genes and NK receptor genes [4-7]). These observations raise the possibility that other genes might be similarly regulated. Moreover, an interesting possibility is that certain genes might be monoallelically expressed in some cells and biallelically expressed in others. Recently, reports of monoallelic expression of interleukin-2 (IL-2) [8,9] and IL-4. [10,11] have raised rite possibility that the cytokine gene family may be subject to monoallelic expression. Another report suggests that the gene encoding the transcription factor Pax-5, which is involved in B-cell (and cerebellar) development [12,13], is also subject to monoallelic expression [14]. Using a novel single-cell reverse transcription-polymerase chain reaction (RT-PCR) approach, we have analyzed the IL-2 and Pax-5 genes in mice. We found that IL-2 is monoallelically transcribed in some T cells and biallelically transcribed in others, raising interesting questions regarding cytokine gene regulation. Additionally, our analyses suggest that Pax-5 is consistently biallelically transcribed. Thus, rite IL-2 gene and other cytokine genes may be regulated in a stochastic manner that results in 0, 1 or 2 alleles of a given cytokine gene expressed in each T cell. This type of regulation could account for the wide variety of different combinations of cytokine genes expressed in individual T cells and therefore plays a role in the generation of T cells with a range of different effector functions.

Original languageAmerican English
Pages (from-to)789-792
Number of pages4
JournalCurrent Biology
Volume10
Issue number13
DOIs
StatePublished - 1 Jun 2000
Externally publishedYes

Bibliographical note

Funding Information:
We thank Y. Bergman, R. Jaenisch, Q. Gao and P. Sklar for helpful discussions and comments on the manuscript and A. Harry for help in manuscript preparation. K.L.R. was supported by NRSA 5 F32 CA68727. This work was supported by a grant from the Ira W. DeCamp Foundation (A.C.). A.C. is a Rita Allen Foundation Scholar.

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