Alleviation of methyl viologen-mediated oxidative stress by Brassica juncea annexin-3 in transgenic Arabidopsis

Ahan Dalal; Abhay Kumar; Deepanker Yadav; Triveni Gudla; Andrea Viehhauser; Karl Josef Dietz; Pulugurtha Bharadwaja Kirti

doi:10.1016/j.plantsci.2013.12.016

Alleviation of methyl viologen-mediated oxidative stress by Brassica juncea annexin-3 in transgenic Arabidopsis

Ahan Dalal^*, Abhay Kumar, Deepanker Yadav, Triveni Gudla, Andrea Viehhauser, Karl Josef Dietz, Pulugurtha Bharadwaja Kirti

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Plant annexins function as calcium-dependent or -independent phospholipid binding proteins and constitute about 0.1% of total cellular proteins. Some of them were reported to antagonize oxidative stress and protect plant cells. Brassica juncea annexin-3 (AnnBj3) was recently discovered. To gain insight into a possible function of AnnBj3 in oxidative stress response, we investigated the resistance of Arabidopsis thaliana plants expressing AnnBj3 constitutively. Here we report that, AnnBj3 attenuates methyl viologen-mediated oxidative stress in plants. It protected photosynthesis and plasma membrane from methyl viologen-mediated oxidative damage. AnnBj3 detoxifies hydrogen peroxide and showed antioxidative property in vitro. The protein increased total peroxidase activity in transgenics and interfered with other cellular antioxidants, thereby giving an overall cellular protection against methyl viologen-induced cytotoxicity.

Original language	English
Pages (from-to)	9-18
Number of pages	10
Journal	Plant Science
Volume	219-220
DOIs	https://doi.org/10.1016/j.plantsci.2013.12.016
State	Published - Apr 2014
Externally published	Yes

Bibliographical note

Funding Information:
PBK acknowledges the grant in the form of a research project from the University Grants Commission, India . The research facilities under DST-FIST and DBT-CREBB in School of Life Sciences, University of Hyderabad, India, are acknowledged. AD is grateful to University of Hyderabad, India and Deutscher Akademischer Austausch Dienst, Germany , for research fellowships and travel grants. AK acknowledges the laboratory facilities of Prof. M.N.V. Prasad, Department of Plant Sciences, University of Hyderabad, India. KJD acknowledges support by the German Science Foundation ( DFG, Di 346 ). Dr. K. Padmasree, Department of Biotechnology and Bioinformatics, University of Hyderabad, India, is greatly acknowledged for CSD1 and 18S rRNA primers. Ms. M. Nalini from Center for Nanotechnology, University of Hyderabad, India, is gratefully acknowledged for her technical expertise in confocal microscopy.

Keywords

Antioxidative property
Brassica juncea annexin-3
Membrane integrity
Methyl viologen
Photosynthetic performance
Redox buffer

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10.1016/j.plantsci.2013.12.016

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title = "Alleviation of methyl viologen-mediated oxidative stress by Brassica juncea annexin-3 in transgenic Arabidopsis",

abstract = "Plant annexins function as calcium-dependent or -independent phospholipid binding proteins and constitute about 0.1% of total cellular proteins. Some of them were reported to antagonize oxidative stress and protect plant cells. Brassica juncea annexin-3 (AnnBj3) was recently discovered. To gain insight into a possible function of AnnBj3 in oxidative stress response, we investigated the resistance of Arabidopsis thaliana plants expressing AnnBj3 constitutively. Here we report that, AnnBj3 attenuates methyl viologen-mediated oxidative stress in plants. It protected photosynthesis and plasma membrane from methyl viologen-mediated oxidative damage. AnnBj3 detoxifies hydrogen peroxide and showed antioxidative property in vitro. The protein increased total peroxidase activity in transgenics and interfered with other cellular antioxidants, thereby giving an overall cellular protection against methyl viologen-induced cytotoxicity.",

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note = "Funding Information: PBK acknowledges the grant in the form of a research project from the University Grants Commission, India . The research facilities under DST-FIST and DBT-CREBB in School of Life Sciences, University of Hyderabad, India, are acknowledged. AD is grateful to University of Hyderabad, India and Deutscher Akademischer Austausch Dienst, Germany , for research fellowships and travel grants. AK acknowledges the laboratory facilities of Prof. M.N.V. Prasad, Department of Plant Sciences, University of Hyderabad, India. KJD acknowledges support by the German Science Foundation ( DFG, Di 346 ). Dr. K. Padmasree, Department of Biotechnology and Bioinformatics, University of Hyderabad, India, is greatly acknowledged for CSD1 and 18S rRNA primers. Ms. M. Nalini from Center for Nanotechnology, University of Hyderabad, India, is gratefully acknowledged for her technical expertise in confocal microscopy. ",

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AU - Viehhauser, Andrea

AU - Dietz, Karl Josef

AU - Kirti, Pulugurtha Bharadwaja

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N2 - Plant annexins function as calcium-dependent or -independent phospholipid binding proteins and constitute about 0.1% of total cellular proteins. Some of them were reported to antagonize oxidative stress and protect plant cells. Brassica juncea annexin-3 (AnnBj3) was recently discovered. To gain insight into a possible function of AnnBj3 in oxidative stress response, we investigated the resistance of Arabidopsis thaliana plants expressing AnnBj3 constitutively. Here we report that, AnnBj3 attenuates methyl viologen-mediated oxidative stress in plants. It protected photosynthesis and plasma membrane from methyl viologen-mediated oxidative damage. AnnBj3 detoxifies hydrogen peroxide and showed antioxidative property in vitro. The protein increased total peroxidase activity in transgenics and interfered with other cellular antioxidants, thereby giving an overall cellular protection against methyl viologen-induced cytotoxicity.

AB - Plant annexins function as calcium-dependent or -independent phospholipid binding proteins and constitute about 0.1% of total cellular proteins. Some of them were reported to antagonize oxidative stress and protect plant cells. Brassica juncea annexin-3 (AnnBj3) was recently discovered. To gain insight into a possible function of AnnBj3 in oxidative stress response, we investigated the resistance of Arabidopsis thaliana plants expressing AnnBj3 constitutively. Here we report that, AnnBj3 attenuates methyl viologen-mediated oxidative stress in plants. It protected photosynthesis and plasma membrane from methyl viologen-mediated oxidative damage. AnnBj3 detoxifies hydrogen peroxide and showed antioxidative property in vitro. The protein increased total peroxidase activity in transgenics and interfered with other cellular antioxidants, thereby giving an overall cellular protection against methyl viologen-induced cytotoxicity.

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Alleviation of methyl viologen-mediated oxidative stress by Brassica juncea annexin-3 in transgenic Arabidopsis

Abstract

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Keywords

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