Alterations in micro-ribonucleic acid expression profiles reveal a novel pathway for estrogen regulation

Amit Cohen, Michael Shmoish, Liraz Levi, Uta Cheruti, Berta Levavi-Sivan, Esther Lubzens*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

58 Scopus citations


Estrogens are steroid hormones that have been implicated in a variety of cellular and physiological processes in the development of diseases such as cancer and are also known to be associated with the effects of endocrine disrupting chemicals. Here we show that 17β-estradiol (E2) alters microRNA (miRNA) expression profiles in the adult zebrafish (Danio rerio). An association between E2 and the expression of 25 miRNAs was found 12 h after treatment. Among the most up-regulated miRNAs were miR-196b and let-7h, and the most downregulated miRNAs included miR-130c and miR-101a. Tissuespecific changes in the transcripts levels of estrogen receptors (Esr1, Esr2a, and Esr2b) and miRNAs were found after hormone treatment. The most up-regulated miR-196b and its precursors are highly expressed in the skin and showed similar tissue-specific expression patterns after treatment, indicating a common pattern of regulation by E2. MiR-196b was shown to fine-tune the expression of its target gene Hoxb8a after treatment in whole-body homogenates. Taken together, our results suggest a novel pathway for the multifunctional and pleiotropic effects of estrogens and open new directions for future investigations of their association with miRNAs involved in estrogen-regulated physiological processes and diseases.

Original languageAmerican English
Pages (from-to)1687-1696
Number of pages10
Issue number4
StatePublished - Apr 2008


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