Alternative splicing and neuritic mRNA translocation under long-term neuronal hypersensitivity

Eran Meshorer, Christina Erb, Roi Gazit, Lev Pavlovsky, Daniela Kaufer, Alon Friedman, David Glick, Nissim Ben-Arie, Hermona Soreq*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

207 Scopus citations

Abstract

To explore neuronal mechanisms underlying long-term consequences of stress, we studied stress-induced changes in the neuritic translocation of acetylcholinesterase (AChE) splice variants. Under normal conditions, we found the synaptic AChE-S mRNA and protein in neurites. Corticosterone, anticholinesterases, and forced swim, each facilitated a rapid (minutes), yet long-lasting (weeks), shift from AChE-S to the normally rare AChE-R mRNA, promoted AChE-R mRNA translocation into neurites, and induced enzyme secretion. Weeks after stress, electrophysiological measurements in hippocampus slices displayed apparently normal evoked synaptic responses but extreme hypersensitivity to both anticholinesterases and atropine. Our findings suggest that neuronal hypersensitivity under stress involves neuritic replacement of AChE-S with AChE-R.

Original languageAmerican English
Pages (from-to)508-512
Number of pages5
JournalScience
Volume295
Issue number5554
DOIs
StatePublished - 18 Jan 2002

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