TY - JOUR
T1 - Alumina nanoparticle-assisted enzyme refolding
T2 - A versatile methodology for proteins renaturation
AU - Volodina, Katerina V.
AU - Avnir, David
AU - Vinogradov, Vladimir V.
N1 - Publisher Copyright:
© 2017 The Author(s).
PY - 2017/12/1
Y1 - 2017/12/1
N2 - We present a high-yield method for the renaturation of negatively charged enzymes. The approach is based on the use of alumina nanoparticles, which after electrostatic interaction with denatured protein molecules, prevent their aggregation and make the process of refolding controllable. The method, demonstrated by the renaturation of several enzymes, is efficient, rapid, employs a minimal amount of reagents and even can be applied to renature mixture of the denatured enzymes.
AB - We present a high-yield method for the renaturation of negatively charged enzymes. The approach is based on the use of alumina nanoparticles, which after electrostatic interaction with denatured protein molecules, prevent their aggregation and make the process of refolding controllable. The method, demonstrated by the renaturation of several enzymes, is efficient, rapid, employs a minimal amount of reagents and even can be applied to renature mixture of the denatured enzymes.
UR - http://www.scopus.com/inward/record.url?scp=85018789180&partnerID=8YFLogxK
U2 - 10.1038/s41598-017-01436-6
DO - 10.1038/s41598-017-01436-6
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C2 - 28469182
AN - SCOPUS:85018789180
SN - 2045-2322
VL - 7
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 1458
ER -