Amino acid sequence of bovine heart coupling factor 6

J. K. Fang, J. W. Jacobs, B. I. Kanner, E. Racker, R. A. Bradshaw

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25 Scopus citations

Abstract

The amino acid sequence of bovine heart mitochondrial coupling factor 6 (F6) has been determined by automated Edman degradation of the whole protein and derived peptides. Preparations based on heat precipitation and ethanol extraction showed allotypic variation at three positions while material further purified by HPLC yielded only one sequence that also differed by a Phe-Thr replacement at residue 62. The mature protein contains 76 amino acids with a calculated molecular weight of 9006 and a pI of ~5, in good agreement with experimentally measured values. The charged amino acids are mainly clustered at the termini and in one section in the middle; these three polar segments are separated by two segments relatively rich in nonpolar residues. Chou-Fasman analysis suggests three stretches of α-helix coinciding (or within) the high-charge-density sequences with a single β-turn at the first polar-nonpolar junction. Comparison of the F6 sequence with those of other proteins did not reveal any homologous structures.

Original languageEnglish
Pages (from-to)6603-6607
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number21 I
DOIs
StatePublished - 1984
Externally publishedYes

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