TY - JOUR
T1 - Amorphous calcium phosphate is a major component of the forming fin bones of zebrafish
T2 - Indications for an amorphous precursor phase
AU - Mahamid, Julia
AU - Sharir, Amnon
AU - Addadi, Lia
AU - Weiner, Steve
PY - 2008/9/2
Y1 - 2008/9/2
N2 - A fundamental question in biomineralization is the nature of the first-formed mineral phase. In vertebrate bone formation, this issue has been the subject of a long-standing controversy. We address this key issue using the continuously growing fin bony rays of the Tuebingen long-fin zebrafish as a model for bone mineralization. Employing high-resolution scanning and transmission electron microscopy imaging, electron diffraction, and elemental analysis, we demonstrate the presence of an abundant amorphous calcium phosphate phase in the newly formed fin bones. The extracted amorphous mineral particles crystallize with time, and mineral crystallinity increases during bone maturation. Based on these findings, we propose that this amorphous calcium phosphate phase may be a precursor phase that later transforms into the mature crystalline mineral.
AB - A fundamental question in biomineralization is the nature of the first-formed mineral phase. In vertebrate bone formation, this issue has been the subject of a long-standing controversy. We address this key issue using the continuously growing fin bony rays of the Tuebingen long-fin zebrafish as a model for bone mineralization. Employing high-resolution scanning and transmission electron microscopy imaging, electron diffraction, and elemental analysis, we demonstrate the presence of an abundant amorphous calcium phosphate phase in the newly formed fin bones. The extracted amorphous mineral particles crystallize with time, and mineral crystallinity increases during bone maturation. Based on these findings, we propose that this amorphous calcium phosphate phase may be a precursor phase that later transforms into the mature crystalline mineral.
KW - Biomineralization
KW - Fish fin
UR - http://www.scopus.com/inward/record.url?scp=51349126458&partnerID=8YFLogxK
U2 - 10.1073/pnas.0803354105
DO - 10.1073/pnas.0803354105
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C2 - 18753619
AN - SCOPUS:51349126458
SN - 0027-8424
VL - 105
SP - 12748
EP - 12753
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 35
ER -