TY - JOUR
T1 - Amperometric amplification of antigen-antibody association at monolayer interfaces
T2 - Design of immunosensor electrodes
AU - Katz, Eugenii
AU - Willner, Itamar
PY - 1996/11/21
Y1 - 1996/11/21
N2 - A mixed-monolayer of an antigen and electrocatalyst assembled onto Au surfaces acts as an immunosensor electrode for the amplified amperometric detection of the complementary antibody (Ab). In the presence of a substrate for the electrocatalyst, the mixed monolayer electrode yields an electrocatalytic current which is blocked by association of the Ab to the monolayer. A mixed monolayer of Nε-2,4-dinitrophenyl lysine and microperoxidase-11 assembled on an Au electrode is applied to the amperometric detection of the dinitrophenyl antibody (DNP-Ab) in the presence of persulfate, S2O2-8, acting as substrate for the microperoxidase electrocatalyst. Similarly, a mixed-monolayer of Nε-2,4-dinitrophenyl lysine and pyrroloquinoline quinone (PQQ) assembled on an Au surface acts as an immunosensing electrode for the DNP-Ab in the presence of 1,4-dihydronicotinamide adenine dinucleotide (NADH) and Ca2+ ions. The electrocatalytic oxidation of NADH in the presence of Ca2+ ions by the PQQ electrocatalyst, and the inhibition of the electrocatalyzed process upon association of the DNP-Ab to the electrode, are used in the analytical sensing of the DNP-Ab. The electrode is sensitive for determination of the DNP-Ab in the concentration range 0.2-20 μ g ml-1.
AB - A mixed-monolayer of an antigen and electrocatalyst assembled onto Au surfaces acts as an immunosensor electrode for the amplified amperometric detection of the complementary antibody (Ab). In the presence of a substrate for the electrocatalyst, the mixed monolayer electrode yields an electrocatalytic current which is blocked by association of the Ab to the monolayer. A mixed monolayer of Nε-2,4-dinitrophenyl lysine and microperoxidase-11 assembled on an Au electrode is applied to the amperometric detection of the dinitrophenyl antibody (DNP-Ab) in the presence of persulfate, S2O2-8, acting as substrate for the microperoxidase electrocatalyst. Similarly, a mixed-monolayer of Nε-2,4-dinitrophenyl lysine and pyrroloquinoline quinone (PQQ) assembled on an Au surface acts as an immunosensing electrode for the DNP-Ab in the presence of 1,4-dihydronicotinamide adenine dinucleotide (NADH) and Ca2+ ions. The electrocatalytic oxidation of NADH in the presence of Ca2+ ions by the PQQ electrocatalyst, and the inhibition of the electrocatalyzed process upon association of the DNP-Ab to the electrode, are used in the analytical sensing of the DNP-Ab. The electrode is sensitive for determination of the DNP-Ab in the concentration range 0.2-20 μ g ml-1.
KW - Amperometric amplification
KW - Antigen monolayer electrode
KW - Dinitrophenyl antibody
KW - Immunosensors
UR - http://www.scopus.com/inward/record.url?scp=0030289184&partnerID=8YFLogxK
U2 - 10.1016/S0022-0728(96)04855-3
DO - 10.1016/S0022-0728(96)04855-3
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AN - SCOPUS:0030289184
SN - 1572-6657
VL - 418
SP - 67
EP - 72
JO - Journal of Electroanalytical Chemistry
JF - Journal of Electroanalytical Chemistry
IS - 1-2
ER -