Amperometric amplification of antigen-antibody association at monolayer interfaces: Design of immunosensor electrodes

Eugenii Katz, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

A mixed-monolayer of an antigen and electrocatalyst assembled onto Au surfaces acts as an immunosensor electrode for the amplified amperometric detection of the complementary antibody (Ab). In the presence of a substrate for the electrocatalyst, the mixed monolayer electrode yields an electrocatalytic current which is blocked by association of the Ab to the monolayer. A mixed monolayer of Nε-2,4-dinitrophenyl lysine and microperoxidase-11 assembled on an Au electrode is applied to the amperometric detection of the dinitrophenyl antibody (DNP-Ab) in the presence of persulfate, S2O2-8, acting as substrate for the microperoxidase electrocatalyst. Similarly, a mixed-monolayer of Nε-2,4-dinitrophenyl lysine and pyrroloquinoline quinone (PQQ) assembled on an Au surface acts as an immunosensing electrode for the DNP-Ab in the presence of 1,4-dihydronicotinamide adenine dinucleotide (NADH) and Ca2+ ions. The electrocatalytic oxidation of NADH in the presence of Ca2+ ions by the PQQ electrocatalyst, and the inhibition of the electrocatalyzed process upon association of the DNP-Ab to the electrode, are used in the analytical sensing of the DNP-Ab. The electrode is sensitive for determination of the DNP-Ab in the concentration range 0.2-20 μ g ml-1.

Original languageEnglish
Pages (from-to)67-72
Number of pages6
JournalJournal of Electroanalytical Chemistry
Volume418
Issue number1-2
DOIs
StatePublished - 21 Nov 1996

Keywords

  • Amperometric amplification
  • Antigen monolayer electrode
  • Dinitrophenyl antibody
  • Immunosensors

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