TY - JOUR
T1 - Amphotericin B-loaded nanoparticles for local treatment of cutaneous leishmaniasis
AU - Abu Ammar, Aiman
AU - Nasereddin, Abed
AU - Ereqat, Suheir
AU - Dan-Goor, Mary
AU - Jaffe, Charles L.
AU - Zussman, Eyal
AU - Abdeen, Ziad
N1 - Publisher Copyright:
© 2018, Controlled Release Society.
PY - 2019/2/15
Y1 - 2019/2/15
N2 - Cutaneous leishmaniasis (CL) is an infectious, parasitic disease caused by the protozoan Leishmania. Amphotericin B (AMB) is a macrolide polyene antibiotic presenting potent antifungal and antileishmanial activity, but due to poor water solubility at physiological pH, side effects, and toxicity, its therapeutic efficiency is limited. In the present study, poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with AMB were generated to reduce drug toxicity and facilitate localized delivery over a prolonged time. AMB NPs were characterized for particle size, zeta potential, polydispersity index, and degree of aggregation. In vitro assessments demonstrated its sustained activity against Leishmania major promastigotes and parasite-infected macrophages. A single intralesional administration to infected BALB/c mice revealed that AMB NPs were more effective than AMB deoxycholate in terms of reducing lesion area. Taken together, these findings suggest that AMB NPs improve AMB delivery and can be used for local treatment of CL.
AB - Cutaneous leishmaniasis (CL) is an infectious, parasitic disease caused by the protozoan Leishmania. Amphotericin B (AMB) is a macrolide polyene antibiotic presenting potent antifungal and antileishmanial activity, but due to poor water solubility at physiological pH, side effects, and toxicity, its therapeutic efficiency is limited. In the present study, poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) loaded with AMB were generated to reduce drug toxicity and facilitate localized delivery over a prolonged time. AMB NPs were characterized for particle size, zeta potential, polydispersity index, and degree of aggregation. In vitro assessments demonstrated its sustained activity against Leishmania major promastigotes and parasite-infected macrophages. A single intralesional administration to infected BALB/c mice revealed that AMB NPs were more effective than AMB deoxycholate in terms of reducing lesion area. Taken together, these findings suggest that AMB NPs improve AMB delivery and can be used for local treatment of CL.
KW - Amphotericin B
KW - Cutaneous leishmaniasis
KW - Nanoparticles
KW - Sustained release
KW - Topical therapy
UR - http://www.scopus.com/inward/record.url?scp=85059828962&partnerID=8YFLogxK
U2 - 10.1007/s13346-018-00603-0
DO - 10.1007/s13346-018-00603-0
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C2 - 30484256
AN - SCOPUS:85059828962
SN - 2190-393X
VL - 9
SP - 76
EP - 84
JO - Drug Delivery and Translational Research
JF - Drug Delivery and Translational Research
IS - 1
ER -