TY - JOUR
T1 - Amplified electronic transduction of oligonucleotide interactions
T2 - Novel routes for Tay-Sachs biosensors
AU - Bardea, Amos
AU - Dagan, Arie
AU - Willner, Itamar
PY - 1999/4/5
Y1 - 1999/4/5
N2 - Two different configurations for the amplified microgravimetric analysis of oligonucleotides (DNA) are presented. A thiol-tagged oligonucleotide, (1), is assembled on a Au-quartz electrode, and the complementary oligonucleotide (2) is microgravimetrically sensed by the crystal. Formation of the ds-oligonucleotide assembly is amplified using mouse anti-dsDNA antibody and goat anti-mouse Fc antibody. Alternatively, a biotin-labeled oligonucleotide, (4), is used to form the ds-oligonucleotide. Biotin is used to amplify the formation of the ds-complex. An electrochemical method, using cytochrome c as redox probe, to assay the formation of the ds-oligonucleotide assembly, is described. The 5'-carboxylic acid-functionalized oligonucleotide (5) is coupled to a cystamine monolayer associated with an Au-electrode. The electrostatic attraction of cytochrome c to the ds-oligonucleotide complex facilitates the interfacial electron transfer to cytochrome c. Formation of the ds-oligonucleotide assembly is detected electrochemically using differential pulse voltammetry. Copyright (C) 1999 Elsevier Science B.V.
AB - Two different configurations for the amplified microgravimetric analysis of oligonucleotides (DNA) are presented. A thiol-tagged oligonucleotide, (1), is assembled on a Au-quartz electrode, and the complementary oligonucleotide (2) is microgravimetrically sensed by the crystal. Formation of the ds-oligonucleotide assembly is amplified using mouse anti-dsDNA antibody and goat anti-mouse Fc antibody. Alternatively, a biotin-labeled oligonucleotide, (4), is used to form the ds-oligonucleotide. Biotin is used to amplify the formation of the ds-complex. An electrochemical method, using cytochrome c as redox probe, to assay the formation of the ds-oligonucleotide assembly, is described. The 5'-carboxylic acid-functionalized oligonucleotide (5) is coupled to a cystamine monolayer associated with an Au-electrode. The electrostatic attraction of cytochrome c to the ds-oligonucleotide complex facilitates the interfacial electron transfer to cytochrome c. Formation of the ds-oligonucleotide assembly is detected electrochemically using differential pulse voltammetry. Copyright (C) 1999 Elsevier Science B.V.
KW - Anti-dsDNA antibody
KW - Biotin-labeled oligonucleotide
KW - DNA-sensor
KW - Electrochemical DNA-sensor
KW - Oligonucleotide monolayer
KW - Quartz-crystal-microbalance
KW - Tay-Sachs
UR - http://www.scopus.com/inward/record.url?scp=0033038440&partnerID=8YFLogxK
U2 - 10.1016/S0003-2670(98)00666-7
DO - 10.1016/S0003-2670(98)00666-7
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AN - SCOPUS:0033038440
SN - 0003-2670
VL - 385
SP - 33
EP - 43
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1-3
ER -