Abstract
Quantum dots (QDs) functionalized with a black-hole quencher are used as optical tracer for the detection of DNA using exonuclease as a biocatalyst. The binding of the target DNA or of a target/open hairpin complex to the functionalized QDs leads to the exonuclease-stimulated recycling of the target DNA or the target/hairpin complex. This results in the triggering of the luminescence of the QDs that provides a readout signal for the amplified sensing process. By using different-sized QDs, the multiplexed detection of DNAs is demonstrated.
Original language | English |
---|---|
Pages (from-to) | 4456-4461 |
Number of pages | 6 |
Journal | Nano Letters |
Volume | 11 |
Issue number | 10 |
DOIs | |
State | Published - 12 Oct 2011 |
Keywords
- DNA
- exonuclease
- FRET
- multiplexed analysis
- Quantum dots
- sensor