AMPLIFIED multiplexed analysis of DNA by the exonuclease iii-catalyzed regeneration of the target dna in the presence of functionalized semiconductor quantum dots

Ronit Freeman, Xiaoqing Liu, Itamar Willner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

161 Scopus citations

Abstract

Quantum dots (QDs) functionalized with a black-hole quencher are used as optical tracer for the detection of DNA using exonuclease as a biocatalyst. The binding of the target DNA or of a target/open hairpin complex to the functionalized QDs leads to the exonuclease-stimulated recycling of the target DNA or the target/hairpin complex. This results in the triggering of the luminescence of the QDs that provides a readout signal for the amplified sensing process. By using different-sized QDs, the multiplexed detection of DNAs is demonstrated.

Original languageEnglish
Pages (from-to)4456-4461
Number of pages6
JournalNano Letters
Volume11
Issue number10
DOIs
StatePublished - 12 Oct 2011

Keywords

  • DNA
  • exonuclease
  • FRET
  • multiplexed analysis
  • Quantum dots
  • sensor

Fingerprint

Dive into the research topics of 'AMPLIFIED multiplexed analysis of DNA by the exonuclease iii-catalyzed regeneration of the target dna in the presence of functionalized semiconductor quantum dots'. Together they form a unique fingerprint.

Cite this