AMPLIFIED multiplexed analysis of DNA by the exonuclease iii-catalyzed regeneration of the target dna in the presence of functionalized semiconductor quantum dots

Ronit Freeman; Xiaoqing Liu; Itamar Willner

doi:10.1021/nl202761g

AMPLIFIED multiplexed analysis of DNA by the exonuclease iii-catalyzed regeneration of the target dna in the presence of functionalized semiconductor quantum dots

Ronit Freeman, Xiaoqing Liu, Itamar Willner^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

163 Scopus citations

Abstract

Quantum dots (QDs) functionalized with a black-hole quencher are used as optical tracer for the detection of DNA using exonuclease as a biocatalyst. The binding of the target DNA or of a target/open hairpin complex to the functionalized QDs leads to the exonuclease-stimulated recycling of the target DNA or the target/hairpin complex. This results in the triggering of the luminescence of the QDs that provides a readout signal for the amplified sensing process. By using different-sized QDs, the multiplexed detection of DNAs is demonstrated.

Original language	English
Pages (from-to)	4456-4461
Number of pages	6
Journal	Nano Letters
Volume	11
Issue number	10
DOIs	https://doi.org/10.1021/nl202761g
State	Published - 12 Oct 2011

Keywords

DNA
FRET
Quantum dots
exonuclease
multiplexed analysis
sensor

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10.1021/nl202761g

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abstract = "Quantum dots (QDs) functionalized with a black-hole quencher are used as optical tracer for the detection of DNA using exonuclease as a biocatalyst. The binding of the target DNA or of a target/open hairpin complex to the functionalized QDs leads to the exonuclease-stimulated recycling of the target DNA or the target/hairpin complex. This results in the triggering of the luminescence of the QDs that provides a readout signal for the amplified sensing process. By using different-sized QDs, the multiplexed detection of DNAs is demonstrated.",

keywords = "DNA, FRET, Quantum dots, exonuclease, multiplexed analysis, sensor",

author = "Ronit Freeman and Xiaoqing Liu and Itamar Willner",

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AU - Freeman, Ronit

AU - Liu, Xiaoqing

AU - Willner, Itamar

PY - 2011/10/12

Y1 - 2011/10/12

N2 - Quantum dots (QDs) functionalized with a black-hole quencher are used as optical tracer for the detection of DNA using exonuclease as a biocatalyst. The binding of the target DNA or of a target/open hairpin complex to the functionalized QDs leads to the exonuclease-stimulated recycling of the target DNA or the target/hairpin complex. This results in the triggering of the luminescence of the QDs that provides a readout signal for the amplified sensing process. By using different-sized QDs, the multiplexed detection of DNAs is demonstrated.

AB - Quantum dots (QDs) functionalized with a black-hole quencher are used as optical tracer for the detection of DNA using exonuclease as a biocatalyst. The binding of the target DNA or of a target/open hairpin complex to the functionalized QDs leads to the exonuclease-stimulated recycling of the target DNA or the target/hairpin complex. This results in the triggering of the luminescence of the QDs that provides a readout signal for the amplified sensing process. By using different-sized QDs, the multiplexed detection of DNAs is demonstrated.

KW - DNA

KW - FRET

KW - Quantum dots

KW - exonuclease

KW - multiplexed analysis

KW - sensor

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AMPLIFIED multiplexed analysis of DNA by the exonuclease iii-catalyzed regeneration of the target dna in the presence of functionalized semiconductor quantum dots

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