TY - JOUR
T1 - An accelerated mass spectrometric method for measuring myo-inositol in phosphatidylinositol in rat brain
AU - Deutsch, Joseph
AU - Ma, Kaizung
AU - Rapoport, Stanley I.
PY - 2006/3/1
Y1 - 2006/3/1
N2 - A fast and efficient chemical ionization mass spectrometric (CI-GC-MS) method for measuring myo-inositol in phosphatidylinositol (PtdIns) in rat brain has been developed. Previously, quantitation of PtdIns involved the release of the myo-inositol by two enzymatic reactions using phospholipase C and alkaline phosphatase. The hydrolytic action of these enzymes was replaced by using commercially available 48% hydrofluoric acid (HF) at 80°C for 30 min. The process can be carried out on the crude Folch extract of brain phospholipids without prior thin layer chromatography (TLC) purification, thereby significantly increasing the speed of analysis. For quantification, unlabeled myo-inositol, labeled myo- and neo-inositol (internal standard) were converted to acetate derivatives and analyzed by CI-GC-MS.
AB - A fast and efficient chemical ionization mass spectrometric (CI-GC-MS) method for measuring myo-inositol in phosphatidylinositol (PtdIns) in rat brain has been developed. Previously, quantitation of PtdIns involved the release of the myo-inositol by two enzymatic reactions using phospholipase C and alkaline phosphatase. The hydrolytic action of these enzymes was replaced by using commercially available 48% hydrofluoric acid (HF) at 80°C for 30 min. The process can be carried out on the crude Folch extract of brain phospholipids without prior thin layer chromatography (TLC) purification, thereby significantly increasing the speed of analysis. For quantification, unlabeled myo-inositol, labeled myo- and neo-inositol (internal standard) were converted to acetate derivatives and analyzed by CI-GC-MS.
KW - CI-GC-MS
KW - Folch extraction
KW - myo-Inositol
KW - Phosphatidylinositol
KW - Rat brain
UR - http://www.scopus.com/inward/record.url?scp=33344458979&partnerID=8YFLogxK
U2 - 10.1016/j.ijms.2005.11.019
DO - 10.1016/j.ijms.2005.11.019
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AN - SCOPUS:33344458979
SN - 1387-3806
VL - 249-250
SP - 392
EP - 395
JO - International Journal of Mass Spectrometry
JF - International Journal of Mass Spectrometry
ER -