An alternative pathway to plant cold tolerance in the absence of vacuolar invertase activity

Paula Teper-Bamnolker, Marina Roitman, Omri Katar, Noam Peleg, Kalaivani Aruchamy, Shlomit Suher, Adi Doron-Faigenboim, Diana Leibman, Ayelet Omid, Eduard Belausov, Mariette Andersson, Niklas Olsson, Ann Sofie Fält, Hanne Volpin, Per Hofvander, Amit Gal-On, Dani Eshel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

To cope with cold stress, plants have developed antioxidation strategies combined with osmoprotection by sugars. In potato (Solanum tuberosum) tubers, which are swollen stems, exposure to cold stress induces starch degradation and sucrose synthesis. Vacuolar acid invertase (VInv) activity is a significant part of the cold-induced sweetening (CIS) response, by rapidly cleaving sucrose into hexoses and increasing osmoprotection. To discover alternative plant tissue pathways for coping with cold stress, we produced VInv-knockout lines in two cultivars. Genome editing of VInv in ‘Désirée’ and ‘Brooke’ was done using stable and transient expression of CRISPR/Cas9 components, respectively. After storage at 4°C, sugar analysis indicated that the knockout lines showed low levels of CIS and maintained low acid invertase activity in storage. Surprisingly, the tuber parenchyma of vinv lines exhibited significantly reduced lipid peroxidation and reduced H2O2 levels. Furthermore, whole plants of vinv lines exposed to cold stress without irrigation showed normal vigor, in contrast to WT plants, which wilted. Transcriptome analysis of vinv lines revealed upregulation of an osmoprotectant pathway and ethylene-related genes during cold temperature exposure. Accordingly, higher expression of antioxidant-related genes was detected after exposure to short and long cold storage. Sugar measurements showed an elevation of an alternative pathway in the absence of VInv activity, raising the raffinose pathway with increasing levels of myo-inositol content as a cold tolerance response.

Original languageAmerican English
Pages (from-to)327-341
Number of pages15
JournalPlant Journal
Volume113
Issue number2
DOIs
StatePublished - Jan 2023
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.

Keywords

  • CRISPR/Cas9
  • Solanum tuberosum
  • cold stress
  • cold-induced sweetening
  • potato
  • transcriptome
  • vacuolar invertase

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