An autonomous bioluminescent bacterial biosensor module for outdoor sensor networks, and its application for the detection of buried explosives

Aharon J. Agranat; Yossef Kabessa; Benjamin Shemer; Etai Shpigel; Offer Schwartsglass; Loay Atamneh; Yonatan Uziel; Meir Ejzenberg; Yosef Mizrachi; Yehudit Garcia; Galina Perepelitsa; Shimshon Belkin

doi:10.1016/j.bios.2021.113253

An autonomous bioluminescent bacterial biosensor module for outdoor sensor networks, and its application for the detection of buried explosives

Aharon J. Agranat, Yossef Kabessa^*, Benjamin Shemer, Etai Shpigel, Offer Schwartsglass, Loay Atamneh, Yonatan Uziel, Meir Ejzenberg, Yosef Mizrachi, Yehudit Garcia, Galina Perepelitsa, Shimshon Belkin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We describe a miniaturized field-deployable biosensor module, designed to function as an element in a sensor network for standoff monitoring and mapping of environmental hazards. The module harbors live bacterial sensor cells, genetically engineered to emit a bioluminescent signal in the presence of preselected target materials, which act as its core sensing elements. The module, which detects and processes the biological signal, composes a digital record that describes its findings, and can be transmitted to a remote receiver. The module is an autonomous self-contained unit that can function either as a standalone sensor, or as a node in a sensor network. The biosensor module can potentially be used for detecting any target material to which the sensor cells were engineered to respond. The module described herein was constructed to detect the presence of buried landmines underneath its footprint. The demonstrated detection sensitivity was 0.25 mg 2,4-dinitrotoluene per Kg soil.

Original language	American English
Article number	113253
Journal	Biosensors and Bioelectronics
Volume	185
DOIs	https://doi.org/10.1016/j.bios.2021.113253
State	Published - 1 Aug 2021

Bibliographical note

Funding Information:
This research was sponsored by the Army Research Office and the Defense Advanced Research Projects Agency ( DARPA ) Biological Technologies Office ( BTO ) and was accomplished under Cooperative Agreement Number W911NF-18-2-0002. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of the Army Research Office and the Defense Advanced Research Projects Agency (DARPA) Biological Technologies Office (BTO) or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation herein. Work in the Belkin lab was partially supported by the Minerva Center for Bio-Hybrid Complex Systems.

Publisher Copyright:
© 2021 Elsevier B.V.

Keywords

Bacterial whole-cell biosensors
Bioluminescence
Chemical sensing
Environmental monitoring
Explosives
Optoelectronic biosensor module

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Agranat, A. J., Kabessa, Y., Shemer, B., Shpigel, E., Schwartsglass, O., Atamneh, L., Uziel, Y., Ejzenberg, M., Mizrachi, Y., Garcia, Y., Perepelitsa, G., & Belkin, S. (2021). An autonomous bioluminescent bacterial biosensor module for outdoor sensor networks, and its application for the detection of buried explosives. Biosensors and Bioelectronics, 185, Article 113253. https://doi.org/10.1016/j.bios.2021.113253

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title = "An autonomous bioluminescent bacterial biosensor module for outdoor sensor networks, and its application for the detection of buried explosives",

abstract = "We describe a miniaturized field-deployable biosensor module, designed to function as an element in a sensor network for standoff monitoring and mapping of environmental hazards. The module harbors live bacterial sensor cells, genetically engineered to emit a bioluminescent signal in the presence of preselected target materials, which act as its core sensing elements. The module, which detects and processes the biological signal, composes a digital record that describes its findings, and can be transmitted to a remote receiver. The module is an autonomous self-contained unit that can function either as a standalone sensor, or as a node in a sensor network. The biosensor module can potentially be used for detecting any target material to which the sensor cells were engineered to respond. The module described herein was constructed to detect the presence of buried landmines underneath its footprint. The demonstrated detection sensitivity was 0.25 mg 2,4-dinitrotoluene per Kg soil.",

keywords = "Bacterial whole-cell biosensors, Bioluminescence, Chemical sensing, Environmental monitoring, Explosives, Optoelectronic biosensor module",

author = "Agranat, {Aharon J.} and Yossef Kabessa and Benjamin Shemer and Etai Shpigel and Offer Schwartsglass and Loay Atamneh and Yonatan Uziel and Meir Ejzenberg and Yosef Mizrachi and Yehudit Garcia and Galina Perepelitsa and Shimshon Belkin",

note = "Funding Information: This research was sponsored by the Army Research Office and the Defense Advanced Research Projects Agency ( DARPA ) Biological Technologies Office ( BTO ) and was accomplished under Cooperative Agreement Number W911NF-18-2-0002. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of the Army Research Office and the Defense Advanced Research Projects Agency (DARPA) Biological Technologies Office (BTO) or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation herein. Work in the Belkin lab was partially supported by the Minerva Center for Bio-Hybrid Complex Systems. Publisher Copyright: {\textcopyright} 2021 Elsevier B.V.",

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doi = "10.1016/j.bios.2021.113253",

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Agranat, AJ, Kabessa, Y, Shemer, B, Shpigel, E, Schwartsglass, O, Atamneh, L, Uziel, Y, Ejzenberg, M, Mizrachi, Y, Garcia, Y, Perepelitsa, G & Belkin, S 2021, 'An autonomous bioluminescent bacterial biosensor module for outdoor sensor networks, and its application for the detection of buried explosives', Biosensors and Bioelectronics, vol. 185, 113253. https://doi.org/10.1016/j.bios.2021.113253

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T1 - An autonomous bioluminescent bacterial biosensor module for outdoor sensor networks, and its application for the detection of buried explosives

AU - Agranat, Aharon J.

AU - Kabessa, Yossef

AU - Shemer, Benjamin

AU - Shpigel, Etai

AU - Schwartsglass, Offer

AU - Atamneh, Loay

AU - Uziel, Yonatan

AU - Ejzenberg, Meir

AU - Mizrachi, Yosef

AU - Garcia, Yehudit

AU - Perepelitsa, Galina

AU - Belkin, Shimshon

N1 - Funding Information: This research was sponsored by the Army Research Office and the Defense Advanced Research Projects Agency ( DARPA ) Biological Technologies Office ( BTO ) and was accomplished under Cooperative Agreement Number W911NF-18-2-0002. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of the Army Research Office and the Defense Advanced Research Projects Agency (DARPA) Biological Technologies Office (BTO) or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation herein. Work in the Belkin lab was partially supported by the Minerva Center for Bio-Hybrid Complex Systems. Publisher Copyright: © 2021 Elsevier B.V.

PY - 2021/8/1

Y1 - 2021/8/1

N2 - We describe a miniaturized field-deployable biosensor module, designed to function as an element in a sensor network for standoff monitoring and mapping of environmental hazards. The module harbors live bacterial sensor cells, genetically engineered to emit a bioluminescent signal in the presence of preselected target materials, which act as its core sensing elements. The module, which detects and processes the biological signal, composes a digital record that describes its findings, and can be transmitted to a remote receiver. The module is an autonomous self-contained unit that can function either as a standalone sensor, or as a node in a sensor network. The biosensor module can potentially be used for detecting any target material to which the sensor cells were engineered to respond. The module described herein was constructed to detect the presence of buried landmines underneath its footprint. The demonstrated detection sensitivity was 0.25 mg 2,4-dinitrotoluene per Kg soil.

AB - We describe a miniaturized field-deployable biosensor module, designed to function as an element in a sensor network for standoff monitoring and mapping of environmental hazards. The module harbors live bacterial sensor cells, genetically engineered to emit a bioluminescent signal in the presence of preselected target materials, which act as its core sensing elements. The module, which detects and processes the biological signal, composes a digital record that describes its findings, and can be transmitted to a remote receiver. The module is an autonomous self-contained unit that can function either as a standalone sensor, or as a node in a sensor network. The biosensor module can potentially be used for detecting any target material to which the sensor cells were engineered to respond. The module described herein was constructed to detect the presence of buried landmines underneath its footprint. The demonstrated detection sensitivity was 0.25 mg 2,4-dinitrotoluene per Kg soil.

KW - Bacterial whole-cell biosensors

KW - Bioluminescence

KW - Chemical sensing

KW - Environmental monitoring

KW - Explosives

KW - Optoelectronic biosensor module

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DO - 10.1016/j.bios.2021.113253

M3 - Article

C2 - 33930754

AN - SCOPUS:85105695770

SN - 0956-5663

VL - 185

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

M1 - 113253

ER -

An autonomous bioluminescent bacterial biosensor module for outdoor sensor networks, and its application for the detection of buried explosives

Abstract

Bibliographical note

Keywords

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