An efficient method for adventitious shoot regeneration from cultured carnation petals

Morly Fisher, Meira Ziv, Alexander Vainstein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


A highly efficient procedure for adventitious shoot regeneration from various parts of carnation petals using liquid media was developed. In contrast to a standard agar-containing medium, in which shoots regenerated from about half of the petals, on the liquid medium shoot regeneration was obtained from nearly all the petals. These shoots regenerated from various parts of the petal, and up to seven adventitious shoots were obtained per petal in cultivar 'White Sim', as well as in other carnation cultivars. The adventitious origin of the regenerated shoots was confirmed by scanning electron microscopy. The use of a liquid media did not affect the extent of vitrification, it being similar to that observed on agar.

Original languageAmerican English
Pages (from-to)231-237
Number of pages7
JournalScientia Horticulturae
Issue number3
StatePublished - Feb 1993

Bibliographical note

Funding Information:
The authors wish to thank R. Shemi Ltd. for providing the plant material and Z. Shmala for her help with the SEM experiments. This work was supported by a grant from the Robert Szold Institute for Applied Science of the P.E.F. Israel Endowment Fund, and the Camation Growers Fund (Association of Israeli Flower Growers). M.F. was supported by the Otto Warburg Center for Biotechnology.


  • Adventitious buds
  • carnation
  • petals


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