An embryonically expressed gene is a target for c-Myc regulation via the c-Myc-binding sequence

Nissim Benvenisty*, Aya Leder, Ann Kuo, Philip Leder

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

151 Scopus citations

Abstract

We have used a subtraction/coexpression strategy involving two different tumors derived from c-myc-bearing transgenic mice to identify a gene that is a target for c-Myc regulation. The gene, expressed in certain embryonic and adult tissues and in several (but not all) c-myc-based tumors, bears a functional c-Myc-binding sequence located 3′ to its transcription start site. This sequence is required for the binding of a nuclear protein complex which, by antibody analysis, includes c-Myc. This site is also required for expression of a reporter gene in chimeric constructs transfected into c-myc-overexpressing cells and, conversely, requires c-myc cotransfection for its enhanced expression in COS cells. Furthermore, transfection of c-myc blocks the normal down-regulation of this gene, which occurs in embryonic stem cells as they undergo differentiation. This target gene encodes an anonymous cDNA (ECA39) found previously to be amplified in a teratocarcinoma cell line.

Original languageAmerican English
Pages (from-to)2513-2523
Number of pages11
JournalGenes and Development
Volume6
Issue number12 PART B
StatePublished - Dec 1992
Externally publishedYes

Keywords

  • DNA-binding
  • Embryo
  • Transformation
  • Transgenic mice
  • c-Myc

Fingerprint

Dive into the research topics of 'An embryonically expressed gene is a target for c-Myc regulation via the c-Myc-binding sequence'. Together they form a unique fingerprint.

Cite this