An enzyme-linked immunosorbent assay for antibodies to Hepatozoon canis

L. Gonen; D. Strauss-Ayali; V. Shkap; N. Vincent-Johnson; D. K. Macintire; G. Baneth

doi:10.1016/j.vetpar.2004.03.021

An enzyme-linked immunosorbent assay for antibodies to Hepatozoon canis

L. Gonen, D. Strauss-Ayali, V. Shkap, N. Vincent-Johnson, D. K. Macintire, G. Baneth^*

^*Corresponding author for this work

The Koret School of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Canine hepatozoonosis is a tick-borne protozoal disease caused in the Old World and South America by Hepatozoon canis. An enzyme-linked immunosorbent assay (ELISA) using purified H. canis gamont antigen was applied for the detection of antibodies reactive with H. canis. Evaluation of the ELISA with sera from naturally infected parasitemic dogs indicated that it was sensitive (86%), specific (97%), and comparable to the indirect fluorescent antibody test (IFAT) for the detection of H. canis antibodies. A variable degree of serologic cross-reactivity was found between sera from H. americanum-infected dogs and the H. canis antigen. Dogs experimentally infected with H. canis seroconverted 1-4 weeks post-infection (PI). Antibody levels peaked at 7-9 weeks PI and gradually declined thereafter remaining above the cut-off value until the conclusion of the study 7 months PI. The ELISA will be valuable for serological evaluation of dogs suspected of exposure to H. canis and for epidemiological studies.

Original language	English
Pages (from-to)	131-139
Number of pages	9
Journal	Veterinary Parasitology
Volume	122
Issue number	2
DOIs	https://doi.org/10.1016/j.vetpar.2004.03.021
State	Published - 21 Jun 2004

Bibliographical note

Funding Information:
The authors thank Dr. Philip H. Kass for his assistance in the statistical analysis of the results. This study was partially funded by Merial Ltd., Lyon, France.

Keywords

ELISA
Hepatozoon americanum
Hepatozoon canis
Rhipicephalus sanguineus

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10.1016/j.vetpar.2004.03.021

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title = "An enzyme-linked immunosorbent assay for antibodies to Hepatozoon canis",

abstract = "Canine hepatozoonosis is a tick-borne protozoal disease caused in the Old World and South America by Hepatozoon canis. An enzyme-linked immunosorbent assay (ELISA) using purified H. canis gamont antigen was applied for the detection of antibodies reactive with H. canis. Evaluation of the ELISA with sera from naturally infected parasitemic dogs indicated that it was sensitive (86%), specific (97%), and comparable to the indirect fluorescent antibody test (IFAT) for the detection of H. canis antibodies. A variable degree of serologic cross-reactivity was found between sera from H. americanum-infected dogs and the H. canis antigen. Dogs experimentally infected with H. canis seroconverted 1-4 weeks post-infection (PI). Antibody levels peaked at 7-9 weeks PI and gradually declined thereafter remaining above the cut-off value until the conclusion of the study 7 months PI. The ELISA will be valuable for serological evaluation of dogs suspected of exposure to H. canis and for epidemiological studies.",

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author = "L. Gonen and D. Strauss-Ayali and V. Shkap and N. Vincent-Johnson and Macintire, {D. K.} and G. Baneth",

note = "Funding Information: The authors thank Dr. Philip H. Kass for his assistance in the statistical analysis of the results. This study was partially funded by Merial Ltd., Lyon, France.",

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AU - Macintire, D. K.

AU - Baneth, G.

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N2 - Canine hepatozoonosis is a tick-borne protozoal disease caused in the Old World and South America by Hepatozoon canis. An enzyme-linked immunosorbent assay (ELISA) using purified H. canis gamont antigen was applied for the detection of antibodies reactive with H. canis. Evaluation of the ELISA with sera from naturally infected parasitemic dogs indicated that it was sensitive (86%), specific (97%), and comparable to the indirect fluorescent antibody test (IFAT) for the detection of H. canis antibodies. A variable degree of serologic cross-reactivity was found between sera from H. americanum-infected dogs and the H. canis antigen. Dogs experimentally infected with H. canis seroconverted 1-4 weeks post-infection (PI). Antibody levels peaked at 7-9 weeks PI and gradually declined thereafter remaining above the cut-off value until the conclusion of the study 7 months PI. The ELISA will be valuable for serological evaluation of dogs suspected of exposure to H. canis and for epidemiological studies.

AB - Canine hepatozoonosis is a tick-borne protozoal disease caused in the Old World and South America by Hepatozoon canis. An enzyme-linked immunosorbent assay (ELISA) using purified H. canis gamont antigen was applied for the detection of antibodies reactive with H. canis. Evaluation of the ELISA with sera from naturally infected parasitemic dogs indicated that it was sensitive (86%), specific (97%), and comparable to the indirect fluorescent antibody test (IFAT) for the detection of H. canis antibodies. A variable degree of serologic cross-reactivity was found between sera from H. americanum-infected dogs and the H. canis antigen. Dogs experimentally infected with H. canis seroconverted 1-4 weeks post-infection (PI). Antibody levels peaked at 7-9 weeks PI and gradually declined thereafter remaining above the cut-off value until the conclusion of the study 7 months PI. The ELISA will be valuable for serological evaluation of dogs suspected of exposure to H. canis and for epidemiological studies.

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An enzyme-linked immunosorbent assay for antibodies to Hepatozoon canis

Abstract

Bibliographical note

Keywords

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