An ex vivo bacteriologic study comparing antiseptic techniques for natural orifice translumenal endoscopic surgery (NOTES) via the gastrointestinal tract

Marvin Ryou, Ronen Hazan, Laurence Rahme, Christopher C. Thompson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Background NOTES via the gastrointestinal tract raises the specter of intra-peritoneal infection. Various antimicrobial techniques have been employed in animal and human survival studies, including saline lavage, intravenous and topical antibiotics, and povidone-iodine, although there is a paucity of data regarding their general effectiveness. Aim To assess the effectiveness of existing sterilization techniques for NOTES by quantifying and speciating colony- forming units (CFUs) before and after treatment. Design Ex vivo animal studies; bacteriological study. Methods Stomachs and distal colons were harvested en bloc from ten fasted adult white pigs following euthanasia. Half received cefazolin 1 g intravenously prior to killing. Multiple tissue samples were obtained from each resected organ. Each tissue sample was then assigned to one of five treatment arms: (1) normal saline, (2) Betadine, (3) cefazolin/metronidazole suspension, (4) chlorhexidine, (5) no treatment. Fifteen samples were used per arm. After treatment, the mucosal surface of each sample was swabbed and inoculated in normal saline, followed by serial dilutions, which were then plated onto sheep's blood agar plates and incubated under aerobic and anaerobic conditions. CFUs were quantified and speciated. Results Median bacterial density was estimated to be 8.0 × 10 5 CFUs/ml (stomach) and 1.9 × 10 6 CFUs/ml (colon). The predominant organisms were Escherichia coli (stomach) and both E. coli and Enterococcus sp. (colon). Saline and antibiotic suspension lavages caused a 1-log reduction in stomach and colon. Betadine/chlorhexidine lavage resulted in a 4-log reduction. Intravenous antibiotics alone resulted in a 4-log reduction. Combining intravenous antibiotics and either Betadine or chlorhexidine decreased counts to the 10 1 level. By Kruskal-Wallis method, differences were statistically significant (p = 0.001). Conclusions The use of intravenous antibiotics in addition to topical Betadine or chlorhexidine effectively reduced microbial burden in both gastric and colonic mucosa in this porcine model to the 10 1 level.

Original languageAmerican English
Pages (from-to)2130-2136
Number of pages7
JournalDigestive Diseases and Sciences
Volume57
Issue number8
DOIs
StatePublished - Aug 2012
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgments This study was supported by a grant from Olympus and the Natural Orifice Surgery Consortium for Assessment and Research (NOSCAR). The authors would also like to thank Rie Maurer for her assistance with statistical analysis.

Keywords

  • Antiseptic technique
  • Chlorhexidine
  • Endoluminal surgery
  • NOTES
  • Natural orifice translumenal endoscopic surgery
  • Povidone
  • Sterility
  • Transcolonic surgery
  • Transgastric surgery
  • Transvaginal surgery

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