TY - JOUR
T1 - An improved dot immunobinding assay for screening hybridoma supernatants. Non-purified antigen immobilized on nitrocellulose paper discs
AU - Steinitz, M.
AU - Rosén, A.
AU - Klein, G.
PY - 1991/1/24
Y1 - 1991/1/24
N2 - This report describes a modified dot immunobinding assay (DIA) in microplates using a crude mixture of non-purified antigen. Nitrocellulose filter paper discs exposed to the antigen mixture were inserted into the wells and kept in place by a specially constructed device. To test the efficiency of the modification a set of monoclonal antibodies from a mouse immunized with 58 kDa trpE-Bmyc fusion protein were screened. The advantage of this modified method over conventional ELISA is that it permits the use of non-purified antigen for screening large numbers of monoclonal antibodies.
AB - This report describes a modified dot immunobinding assay (DIA) in microplates using a crude mixture of non-purified antigen. Nitrocellulose filter paper discs exposed to the antigen mixture were inserted into the wells and kept in place by a specially constructed device. To test the efficiency of the modification a set of monoclonal antibodies from a mouse immunized with 58 kDa trpE-Bmyc fusion protein were screened. The advantage of this modified method over conventional ELISA is that it permits the use of non-purified antigen for screening large numbers of monoclonal antibodies.
KW - Dot immunobinding assay
KW - Monoclonal antibody
KW - Nitrocellulose paper disc
KW - Non-purified antigen
UR - http://www.scopus.com/inward/record.url?scp=0026008561&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(91)90257-G
DO - 10.1016/0022-1759(91)90257-G
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C2 - 1995705
AN - SCOPUS:0026008561
SN - 0022-1759
VL - 136
SP - 119
EP - 123
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -