An improved dot immunobinding assay for screening hybridoma supernatants. Non-purified antigen immobilized on nitrocellulose paper discs

M. Steinitz*, A. Rosén, G. Klein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

This report describes a modified dot immunobinding assay (DIA) in microplates using a crude mixture of non-purified antigen. Nitrocellulose filter paper discs exposed to the antigen mixture were inserted into the wells and kept in place by a specially constructed device. To test the efficiency of the modification a set of monoclonal antibodies from a mouse immunized with 58 kDa trpE-Bmyc fusion protein were screened. The advantage of this modified method over conventional ELISA is that it permits the use of non-purified antigen for screening large numbers of monoclonal antibodies.

Original languageEnglish
Pages (from-to)119-123
Number of pages5
JournalJournal of Immunological Methods
Volume136
Issue number1
DOIs
StatePublished - 24 Jan 1991
Externally publishedYes

Keywords

  • Dot immunobinding assay
  • Monoclonal antibody
  • Nitrocellulose paper disc
  • Non-purified antigen

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