Abstract
A simple, rapid and effective method for the isolation of the soluble tumor-associated antigens from cancer patient serum is described. The method is based on the use of gel fiberglass (GFG) affinity chromatography and BioGel P-60 columns which enable isolation of single proteins from blood sera. GFG is a new affinity biosensor for isolation of human s53 antigen using rabbit anti-s53 IgG. The described method may be used for the purification of different organ-specific antigens which are of interest in biology and medicine.
| Original language | English |
|---|---|
| Pages (from-to) | 55-58 |
| Number of pages | 4 |
| Journal | Experimental Oncology |
| Volume | 21 |
| Issue number | 1 |
| State | Published - Mar 1999 |
Keywords
- Affinity chromatography
- Colon cancer
- Gel fiberglass
- IgG
- Tumor-associated antigens
