TY - JOUR
T1 - An improved protocol for the preparation of a frozen promastigote vaccine for cutaneous leishmaniasis
AU - Greenblatt, C. L.
AU - Spira, D. T.
AU - Montilio, B.
AU - Gerichter, H.
PY - 1980/7
Y1 - 1980/7
N2 - Until very recently the promastigote vaccine against cutaneous leishmaniasis used in Israel could only be tested simultaneously with its injection into man. A simple protocol for freezing the vaccine in liquid nitrogen is described, which allows prolonged storage of the material in the final container. During storage, tests of biochemical identity, sterility, pyrogenicity and infectivity for animals and cultured cells are carried out. The logistics of transport are discussed. Transfer from -196 to -20°C was deleterious, while storage at -79°C preserved activity for at least a month.
AB - Until very recently the promastigote vaccine against cutaneous leishmaniasis used in Israel could only be tested simultaneously with its injection into man. A simple protocol for freezing the vaccine in liquid nitrogen is described, which allows prolonged storage of the material in the final container. During storage, tests of biochemical identity, sterility, pyrogenicity and infectivity for animals and cultured cells are carried out. The logistics of transport are discussed. Transfer from -196 to -20°C was deleterious, while storage at -79°C preserved activity for at least a month.
UR - http://www.scopus.com/inward/record.url?scp=0019162262&partnerID=8YFLogxK
U2 - 10.1016/S0092-1157(80)80038-2
DO - 10.1016/S0092-1157(80)80038-2
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C2 - 7410446
AN - SCOPUS:0019162262
SN - 0092-1157
VL - 8
SP - 227
EP - 232
JO - Journal of Biological Standardization
JF - Journal of Biological Standardization
IS - 3
ER -