An improved selective medium for the isolation of Verticillium dahliae

R. Ausher*, J. Katan, S. Ovadia

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

A selective medium was developed for the isolation and enumeration of Verticillium dahliae in senescent infected tomato tissues heavily colonized with various fungi, from which the pathogen could not be isolated by the common ethanolstreptomycin selective medium. Suppression of the accompanying saprophytes, especially Fusarium, was achieved by the combined effects of relatively low incubation temperature (18°C) and supplementation of the medium with ethanol (0.5%), pentachloronitrobenzene (50 ppm) and antibiotics. Addition of sucrose (0.75%) and Czapek's salts improved visualization of V. dahliae colonies which appeared black. V. dahliae populations were estimated in senescent infected tissues from various sources by use of this medium.

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalPhytoparasitica
Volume3
Issue number2
DOIs
StatePublished - Dec 1975

Keywords

  • selective medium
  • Verticillium dahliae

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