An integrated genetic linkage map of avocado

D. Sharon; P. B. Cregan; S. Mhameed; M. Kusharska; J. Hillel; E. Lahav; U. Lavi

doi:10.1007/s001220050642

An integrated genetic linkage map of avocado

D. Sharon, P. B. Cregan, S. Mhameed, M. Kusharska, J. Hillel, E. Lahav, U. Lavi^*

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

An avocado genomic library was screened with various microsatellite repeats. (A/T)(n) and (TC/AG)(n) sequences were found to be the most frequent repeats. One hundred and seventy-two positive clones were sequenced successfully of which 113 were found to contain simple sequence repeats (SSR). Polymerase chain reaction primers were designed to the regions flanking the SSR in 62 clones. A GenBank search of avocado DNA sequences revealed 1 sequence containing a (CT)₁₀ repeat. A total of 92 avocado-specific SSR markers were screened for polymorphism using 50 offspring of a cross between the avocado cultivars 'Pinkerton' and 'Ettinger'. Both are standard avocado cultivars which are normally outcrossed and highly heterozygous. Fifty polymorphic SSR loci, 17 random amplified polymorphic DNA (RAPD) and 23 minisatellite DNA Fingerprint (DFP) bands were used to construct the avocado genetic map. The resulting data were analyzed with various mapping programs in order to assess which program best accommodated data from progeny of heterozygous parents. The analyses resulted in 12 linkage groups with 34 markers (25 SSRs, 3 RAPDs and 6 DFP bands) covering 352.6 cM. This initial map can serve as a basis for developing a detailed genomic map and for detection of linkage between markers and quantitative trait loci.

Original language	English
Pages (from-to)	911-921
Number of pages	11
Journal	Theoretical And Applied Genetics
Volume	95
Issue number	5-6
DOIs	https://doi.org/10.1007/s001220050642
State	Published - Oct 1997

Keywords

Linkage groups
Mendelian inheritance Microsatellites
Minisatellites
Simple sequence repeats
Variable number of tandem repeats

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10.1007/s001220050642

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title = "An integrated genetic linkage map of avocado",

abstract = "An avocado genomic library was screened with various microsatellite repeats. (A/T)(n) and (TC/AG)(n) sequences were found to be the most frequent repeats. One hundred and seventy-two positive clones were sequenced successfully of which 113 were found to contain simple sequence repeats (SSR). Polymerase chain reaction primers were designed to the regions flanking the SSR in 62 clones. A GenBank search of avocado DNA sequences revealed 1 sequence containing a (CT)10 repeat. A total of 92 avocado-specific SSR markers were screened for polymorphism using 50 offspring of a cross between the avocado cultivars 'Pinkerton' and 'Ettinger'. Both are standard avocado cultivars which are normally outcrossed and highly heterozygous. Fifty polymorphic SSR loci, 17 random amplified polymorphic DNA (RAPD) and 23 minisatellite DNA Fingerprint (DFP) bands were used to construct the avocado genetic map. The resulting data were analyzed with various mapping programs in order to assess which program best accommodated data from progeny of heterozygous parents. The analyses resulted in 12 linkage groups with 34 markers (25 SSRs, 3 RAPDs and 6 DFP bands) covering 352.6 cM. This initial map can serve as a basis for developing a detailed genomic map and for detection of linkage between markers and quantitative trait loci.",

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author = "D. Sharon and Cregan, {P. B.} and S. Mhameed and M. Kusharska and J. Hillel and E. Lahav and U. Lavi",

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AU - Sharon, D.

AU - Cregan, P. B.

AU - Mhameed, S.

AU - Kusharska, M.

AU - Hillel, J.

AU - Lahav, E.

AU - Lavi, U.

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An integrated genetic linkage map of avocado

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Keywords

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