TY - JOUR
T1 - An isatin β thiosemicarbazone (IBT) dependent mutant of vaccinia virus
T2 - the nature of the IBT dependent step
AU - Katz, E.
AU - Margalith, E.
AU - Winer, B.
PY - 1973
Y1 - 1973
N2 - The IBT dependent mutant of vaccinia virus needs IBT for its growth. IBT is not essential for the synthesis of virus DNA, but the formed DNA does not become resistant to deoxyribonuclease in its absence. Both 'early' and 'late' virus proteins are synthesized in cells infected with the mutant, but in the absence of IBT one of the virus core structural polypeptides, which is normally formed from a higher mol. wt. precursor, is not made. These findings suggest that the IBT dependent step of the mutant occurs earlier in the maturation process of the virus than does the block produced by IBT with the wild type strain.
AB - The IBT dependent mutant of vaccinia virus needs IBT for its growth. IBT is not essential for the synthesis of virus DNA, but the formed DNA does not become resistant to deoxyribonuclease in its absence. Both 'early' and 'late' virus proteins are synthesized in cells infected with the mutant, but in the absence of IBT one of the virus core structural polypeptides, which is normally formed from a higher mol. wt. precursor, is not made. These findings suggest that the IBT dependent step of the mutant occurs earlier in the maturation process of the virus than does the block produced by IBT with the wild type strain.
UR - http://www.scopus.com/inward/record.url?scp=0015865077&partnerID=8YFLogxK
U2 - 10.1099/0022-1317-21-3-477
DO - 10.1099/0022-1317-21-3-477
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C2 - 4763971
AN - SCOPUS:0015865077
SN - 0022-1317
VL - 21
SP - 477
EP - 484
JO - Journal of General Virology
JF - Journal of General Virology
IS - 3
ER -