An N-methyl-d-aspartate (NMDA) receptor antagonist reduces bicuculline-induced depolarization shifts in neocortical explant cultures

F. Baldino; B. Wolfson; U. Heinemann; M. J. Gutnick

doi:10.1016/0304-3940(86)90445-3

An N-methyl-d-aspartate (NMDA) receptor antagonist reduces bicuculline-induced depolarization shifts in neocortical explant cultures

F. Baldino^*, B. Wolfson, U. Heinemann, M. J. Gutnick

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

We investigated the actions of a specific N-methyl-d-aspartate (NMDA) antagonist, 2-amino-5-phosphonovaleric acid (2-APV), on bicuculline-induced epileptogenesis in organotypic explant cultures from neonatal rat neocortex. Explants were maintained in roller tubes for 3-5 weeks. The late, plateau phase of the intracellularly recorded paroxysmal depolarization shift was sensitive to both intracellularly injected hyperpolarizing currents and 2-APV, suggesting that this component is generated by a voltage-dependent, regenerative process that is mediated by activation of NMDA receptors. The results support the hypothesis that NMDA receptors play an important role in the generation of epileptiform activity by localized circuits of neocortical neurons.

Original language	English
Pages (from-to)	101-105
Number of pages	5
Journal	Neuroscience Letters
Volume	70
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(86)90445-3
State	Published - 25 Sep 1986
Externally published	Yes

Keywords

2-Amino-5-phosphonovaleric acid
Depolarization shift
Epileptogenesis
Explant culture
N-Methyl-d-aspartate
Neocortex

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10.1016/0304-3940(86)90445-3

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title = "An N-methyl-d-aspartate (NMDA) receptor antagonist reduces bicuculline-induced depolarization shifts in neocortical explant cultures",

abstract = "We investigated the actions of a specific N-methyl-d-aspartate (NMDA) antagonist, 2-amino-5-phosphonovaleric acid (2-APV), on bicuculline-induced epileptogenesis in organotypic explant cultures from neonatal rat neocortex. Explants were maintained in roller tubes for 3-5 weeks. The late, plateau phase of the intracellularly recorded paroxysmal depolarization shift was sensitive to both intracellularly injected hyperpolarizing currents and 2-APV, suggesting that this component is generated by a voltage-dependent, regenerative process that is mediated by activation of NMDA receptors. The results support the hypothesis that NMDA receptors play an important role in the generation of epileptiform activity by localized circuits of neocortical neurons.",

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AU - Gutnick, M. J.

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An N-methyl-d-aspartate (NMDA) receptor antagonist reduces bicuculline-induced depolarization shifts in neocortical explant cultures

Abstract

Keywords

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