An optical submicrometer calcium sensor with conductance sensing capability

S. Shalom; A. Strinkovski; G. Peleg; S. Druckmann; A. Krauss; A. Lewis; M. Linial; M. Ottolenghi

doi:10.1006/abio.1996.9893

An optical submicrometer calcium sensor with conductance sensing capability

S. Shalom, A. Strinkovski, G. Peleg, S. Druckmann, A. Krauss, A. Lewis, M. Linial, M. Ottolenghi^*

^*Corresponding author for this work

Department of Biological Chemistry

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The identification of chemical species and the measurement of their concentrations with high (submicrometer) spatial resolution are of considerable importance in cell biology. In this article we report the first successful development of a ≤0.1-μm Ca²⁺ sensor based on a pulled micropipet, filled with a conducting porous sol-gel glass which was doped with the fluorescent calcium green 1 Ca²⁺ indicator. Such sensors are potentially capable of measuring Ca²⁺ concentrations as low as 10^-8 M, in confined volumes, with a three-dimensional resolution which exceeds ~0.1 μm. A major advantage of the sensor is its capability to be integrated into a multifunctional probe which will measure chemical analyte concentrations and ion conductance.

Original language	American English
Pages (from-to)	256-259
Number of pages	4
Journal	Analytical Biochemistry
Volume	244
Issue number	2
DOIs	https://doi.org/10.1006/abio.1996.9893
State	Published - 15 Jan 1997

Bibliographical note

Funding Information:
The authors acknowledge support by the Israel National Council for Research and Development. We thank Ms. D. Parnas for valuable assistance with the preparation of the cells.

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10.1006/abio.1996.9893

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abstract = "The identification of chemical species and the measurement of their concentrations with high (submicrometer) spatial resolution are of considerable importance in cell biology. In this article we report the first successful development of a ≤0.1-μm Ca2+ sensor based on a pulled micropipet, filled with a conducting porous sol-gel glass which was doped with the fluorescent calcium green 1 Ca2+ indicator. Such sensors are potentially capable of measuring Ca2+ concentrations as low as 10-8 M, in confined volumes, with a three-dimensional resolution which exceeds ~0.1 μm. A major advantage of the sensor is its capability to be integrated into a multifunctional probe which will measure chemical analyte concentrations and ion conductance.",

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AU - Strinkovski, A.

AU - Peleg, G.

AU - Druckmann, S.

AU - Krauss, A.

AU - Lewis, A.

AU - Linial, M.

AU - Ottolenghi, M.

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AB - The identification of chemical species and the measurement of their concentrations with high (submicrometer) spatial resolution are of considerable importance in cell biology. In this article we report the first successful development of a ≤0.1-μm Ca2+ sensor based on a pulled micropipet, filled with a conducting porous sol-gel glass which was doped with the fluorescent calcium green 1 Ca2+ indicator. Such sensors are potentially capable of measuring Ca2+ concentrations as low as 10-8 M, in confined volumes, with a three-dimensional resolution which exceeds ~0.1 μm. A major advantage of the sensor is its capability to be integrated into a multifunctional probe which will measure chemical analyte concentrations and ion conductance.

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An optical submicrometer calcium sensor with conductance sensing capability

Abstract

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