TY - JOUR
T1 - An optical submicrometer calcium sensor with conductance sensing capability
AU - Shalom, S.
AU - Strinkovski, A.
AU - Peleg, G.
AU - Druckmann, S.
AU - Krauss, A.
AU - Lewis, A.
AU - Linial, M.
AU - Ottolenghi, M.
N1 - Funding Information:
The authors acknowledge support by the Israel National Council for Research and Development. We thank Ms. D. Parnas for valuable assistance with the preparation of the cells.
PY - 1997/1/15
Y1 - 1997/1/15
N2 - The identification of chemical species and the measurement of their concentrations with high (submicrometer) spatial resolution are of considerable importance in cell biology. In this article we report the first successful development of a ≤0.1-μm Ca2+ sensor based on a pulled micropipet, filled with a conducting porous sol-gel glass which was doped with the fluorescent calcium green 1 Ca2+ indicator. Such sensors are potentially capable of measuring Ca2+ concentrations as low as 10-8 M, in confined volumes, with a three-dimensional resolution which exceeds ~0.1 μm. A major advantage of the sensor is its capability to be integrated into a multifunctional probe which will measure chemical analyte concentrations and ion conductance.
AB - The identification of chemical species and the measurement of their concentrations with high (submicrometer) spatial resolution are of considerable importance in cell biology. In this article we report the first successful development of a ≤0.1-μm Ca2+ sensor based on a pulled micropipet, filled with a conducting porous sol-gel glass which was doped with the fluorescent calcium green 1 Ca2+ indicator. Such sensors are potentially capable of measuring Ca2+ concentrations as low as 10-8 M, in confined volumes, with a three-dimensional resolution which exceeds ~0.1 μm. A major advantage of the sensor is its capability to be integrated into a multifunctional probe which will measure chemical analyte concentrations and ion conductance.
UR - http://www.scopus.com/inward/record.url?scp=0031568273&partnerID=8YFLogxK
U2 - 10.1006/abio.1996.9893
DO - 10.1006/abio.1996.9893
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C2 - 9025941
AN - SCOPUS:0031568273
SN - 0003-2697
VL - 244
SP - 256
EP - 259
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -