An upstream repressor element plays a role in Igf2 imprinting

Sharon Eden; Miguel Constancia; Tamar Hashimshony; Wendy Dean; Beth Goldstein; Alfred C. Johnson; Ilana Keshet; Wolf Reik; Howard Cedar

doi:10.1093/emboj/20.13.3518

An upstream repressor element plays a role in Igf2 imprinting

Sharon Eden, Miguel Constancia, Tamar Hashimshony, Wendy Dean, Beth Goldstein, Alfred C. Johnson, Ilana Keshet, Wolf Reik, Howard Cedar^*

^*Corresponding author for this work

Department of Microbiology and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

83 Scopus citations

Abstract

The imprinted Igf2 gene is associated with a small upstream region that is differentially methylated on the active paternal allele. We have identified a repressor element within this sequence and shown that repression is probably mediated through a transacting factor, GCF2. DNA methylation of this site abrogates both protein binding and repressor activity. Targeting experiments demonstrate that this element plays a role in the repression of the maternal Igf2 gene in vivo.

Original language	English
Pages (from-to)	3518-3525
Number of pages	8
Journal	EMBO Journal
Volume	20
Issue number	13
DOIs	https://doi.org/10.1093/emboj/20.13.3518
State	Published - 2 Jul 2001

Keywords

GCF
Imprinting
Methylation

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10.1093/emboj/20.13.3518

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AU - Keshet, Ilana

AU - Reik, Wolf

AU - Cedar, Howard

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AB - The imprinted Igf2 gene is associated with a small upstream region that is differentially methylated on the active paternal allele. We have identified a repressor element within this sequence and shown that repression is probably mediated through a transacting factor, GCF2. DNA methylation of this site abrogates both protein binding and repressor activity. Targeting experiments demonstrate that this element plays a role in the repression of the maternal Igf2 gene in vivo.

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KW - Methylation

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An upstream repressor element plays a role in Igf2 imprinting

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Keywords

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