An x-ray atlas of the sagittal plane of the chicken diencephalon and its use in the precise localization of brain sites

An x-ray atlas of the sagittal plane of the chicken diencephalon, which enables a more precise placement of electrolytic lesions, or the implantation of cannulas and electrodes, was constructed. The localization of brain sites is accomplished on the horizontal and vertical planes only; for the lateral plane coordinates, a previously published complementary atlas is required. A high correlation was found between stereotaxic coordinates and the anticipated locations of electrolytic lesions. A procedure is described, which utilizes radiography with two reference points to determine and facilitate the use of specific brain coordinates.