TY - JOUR
T1 - Analysis of 5-methylcytosine in DNA. I. Mass spectrometry
AU - Deutsch, Joseph
AU - Razin, Aharon
AU - Sedat, John
PY - 1976/5/7
Y1 - 1976/5/7
N2 - A method is described for the unambiguous rapid identification and quantitation of the minor base, 5-methylcytosine, in DNA using high resolution mass spectrometry. This method can detect one 5-methylcytosine residue per 5500 nucleotides in φχ 174 DNA, in a sample of less than 10 μg and requires less than 1 μg of calf thymus DNA in which the molar ratio of 5-methylcytosine/cytosine is about 0.05.
AB - A method is described for the unambiguous rapid identification and quantitation of the minor base, 5-methylcytosine, in DNA using high resolution mass spectrometry. This method can detect one 5-methylcytosine residue per 5500 nucleotides in φχ 174 DNA, in a sample of less than 10 μg and requires less than 1 μg of calf thymus DNA in which the molar ratio of 5-methylcytosine/cytosine is about 0.05.
UR - http://www.scopus.com/inward/record.url?scp=0017158602&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(76)90570-4
DO - 10.1016/0003-2697(76)90570-4
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C2 - 945948
AN - SCOPUS:0017158602
SN - 0003-2697
VL - 72
SP - 586
EP - 592
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1-2
ER -