Analysis of 5-methylcytosine in DNA. I. Mass spectrometry

Joseph Deutsch*, Aharon Razin, John Sedat

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

A method is described for the unambiguous rapid identification and quantitation of the minor base, 5-methylcytosine, in DNA using high resolution mass spectrometry. This method can detect one 5-methylcytosine residue per 5500 nucleotides in φχ 174 DNA, in a sample of less than 10 μg and requires less than 1 μg of calf thymus DNA in which the molar ratio of 5-methylcytosine/cytosine is about 0.05.

Original languageEnglish
Pages (from-to)586-592
Number of pages7
JournalAnalytical Biochemistry
Volume72
Issue number1-2
DOIs
StatePublished - 7 May 1976

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