Analysis of 5-methylcytosine in DNA. I. Mass spectrometry

Joseph Deutsch; Aharon Razin; John Sedat

doi:10.1016/0003-2697(76)90570-4

Analysis of 5-methylcytosine in DNA. I. Mass spectrometry

Joseph Deutsch^*
, Aharon Razin
, John Sedat

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

A method is described for the unambiguous rapid identification and quantitation of the minor base, 5-methylcytosine, in DNA using high resolution mass spectrometry. This method can detect one 5-methylcytosine residue per 5500 nucleotides in φχ 174 DNA, in a sample of less than 10 μg and requires less than 1 μg of calf thymus DNA in which the molar ratio of 5-methylcytosine/cytosine is about 0.05.

Original language	English
Pages (from-to)	586-592
Number of pages	7
Journal	Analytical Biochemistry
Volume	72
Issue number	1-2
DOIs	https://doi.org/10.1016/0003-2697(76)90570-4
State	Published - 7 May 1976

Access to Document

10.1016/0003-2697(76)90570-4

Cite this

@article{fbf074b9fce145d99984074ba492aa8e,

title = "Analysis of 5-methylcytosine in DNA. I. Mass spectrometry",

abstract = "A method is described for the unambiguous rapid identification and quantitation of the minor base, 5-methylcytosine, in DNA using high resolution mass spectrometry. This method can detect one 5-methylcytosine residue per 5500 nucleotides in φχ 174 DNA, in a sample of less than 10 μg and requires less than 1 μg of calf thymus DNA in which the molar ratio of 5-methylcytosine/cytosine is about 0.05.",

author = "Joseph Deutsch and Aharon Razin and John Sedat",

year = "1976",

month = may,

day = "7",

doi = "10.1016/0003-2697(76)90570-4",

language = "אנגלית",

volume = "72",

pages = "586--592",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1-2",

}

TY - JOUR

T1 - Analysis of 5-methylcytosine in DNA. I. Mass spectrometry

AU - Deutsch, Joseph

AU - Razin, Aharon

AU - Sedat, John

PY - 1976/5/7

Y1 - 1976/5/7

N2 - A method is described for the unambiguous rapid identification and quantitation of the minor base, 5-methylcytosine, in DNA using high resolution mass spectrometry. This method can detect one 5-methylcytosine residue per 5500 nucleotides in φχ 174 DNA, in a sample of less than 10 μg and requires less than 1 μg of calf thymus DNA in which the molar ratio of 5-methylcytosine/cytosine is about 0.05.

AB - A method is described for the unambiguous rapid identification and quantitation of the minor base, 5-methylcytosine, in DNA using high resolution mass spectrometry. This method can detect one 5-methylcytosine residue per 5500 nucleotides in φχ 174 DNA, in a sample of less than 10 μg and requires less than 1 μg of calf thymus DNA in which the molar ratio of 5-methylcytosine/cytosine is about 0.05.

UR - https://www.scopus.com/pages/publications/0017158602

U2 - 10.1016/0003-2697(76)90570-4

DO - 10.1016/0003-2697(76)90570-4

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 945948

AN - SCOPUS:0017158602

SN - 0003-2697

VL - 72

SP - 586

EP - 592

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1-2

ER -

Analysis of 5-methylcytosine in DNA. I. Mass spectrometry

Abstract

Access to Document

Other files and links

Fingerprint

Cite this