Analysis of herpes simplex virus DNA synthesized in vitro in nuclei isolated from infected BSC-1 cells

Joseph Shlomai; Yechiel Becker

doi:10.1016/0042-6822(75)90170-1

Analysis of herpes simplex virus DNA synthesized in vitro in nuclei isolated from infected BSC-1 cells

Joseph Shlomai^*, Yechiel Becker

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Herpes simplex virus (HSV) DNA molecules synthesized in isolated nuclei in vitro were analyzed by centrifugation in sucrose gradients and by chromatography on benzoylated naphthoylated DEAE (BND)-cellulose columns. Some of the in vitro synthesized viral DNA molecules were found to have a high molecular weight when cosedimented with a HSV-DNA marker. Over 60% of the in vitro labeled DNA molecules were eluted from the BND-cellulose columns by 1.0 M NaCl containing 2% caffeine, indicating the presence of single-stranded sequences in the DNA molecules, a property of replicating DNA molecules. It is concluded that under in vitro conditions, the elongation of nascent viral DNA molecules takes place.

Original language	English
Pages (from-to)	275-279
Number of pages	5
Journal	Virology
Volume	68
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(75)90170-1
State	Published - Nov 1975

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title = "Analysis of herpes simplex virus DNA synthesized in vitro in nuclei isolated from infected BSC-1 cells",

abstract = "Herpes simplex virus (HSV) DNA molecules synthesized in isolated nuclei in vitro were analyzed by centrifugation in sucrose gradients and by chromatography on benzoylated naphthoylated DEAE (BND)-cellulose columns. Some of the in vitro synthesized viral DNA molecules were found to have a high molecular weight when cosedimented with a HSV-DNA marker. Over 60% of the in vitro labeled DNA molecules were eluted from the BND-cellulose columns by 1.0 M NaCl containing 2% caffeine, indicating the presence of single-stranded sequences in the DNA molecules, a property of replicating DNA molecules. It is concluded that under in vitro conditions, the elongation of nascent viral DNA molecules takes place.",

author = "Joseph Shlomai and Yechiel Becker",

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AB - Herpes simplex virus (HSV) DNA molecules synthesized in isolated nuclei in vitro were analyzed by centrifugation in sucrose gradients and by chromatography on benzoylated naphthoylated DEAE (BND)-cellulose columns. Some of the in vitro synthesized viral DNA molecules were found to have a high molecular weight when cosedimented with a HSV-DNA marker. Over 60% of the in vitro labeled DNA molecules were eluted from the BND-cellulose columns by 1.0 M NaCl containing 2% caffeine, indicating the presence of single-stranded sequences in the DNA molecules, a property of replicating DNA molecules. It is concluded that under in vitro conditions, the elongation of nascent viral DNA molecules takes place.

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Analysis of herpes simplex virus DNA synthesized in vitro in nuclei isolated from infected BSC-1 cells

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