TY - JOUR
T1 - Analysis of myogenesis with recombinant DNA techniques.
AU - Yaffe, D.
AU - Nudel, U.
AU - Czosnek, H.
AU - Zakut, R.
AU - Carmon, Y.
AU - Shani, M.
PY - 1982
Y1 - 1982
N2 - Recombinant phages containing the rat skeletal muscle alpha-actin gene and the cytoplasmic beta-actin gene were isolated and the structure of these genes was determined. Both genes contain a large intron in the 5' untranslated region and smaller introns at codons 41, 267 and 327. In addition, the alpha-actin contains introns at codons 150 and 204 not present in the beta-actin gene, whereas the beta-actin gene contains an intron at codon 121. The evolutionary aspects of these findings are discussed. Active genes are organized in chromatin in a conformation which renders them preferentially sensitive to digestion with nucleolytic enzymes. The DNAase I sensitivity of genes programmed to be expressed during myogenesis was tested in a cloned cell population of a myogenic cell line. It was found that these genes are not preferentially sensitive to DNAase I in the chromatin of proliferating mononucleated cells. They become DNAase I sensitive during terminal differentiation.
AB - Recombinant phages containing the rat skeletal muscle alpha-actin gene and the cytoplasmic beta-actin gene were isolated and the structure of these genes was determined. Both genes contain a large intron in the 5' untranslated region and smaller introns at codons 41, 267 and 327. In addition, the alpha-actin contains introns at codons 150 and 204 not present in the beta-actin gene, whereas the beta-actin gene contains an intron at codon 121. The evolutionary aspects of these findings are discussed. Active genes are organized in chromatin in a conformation which renders them preferentially sensitive to digestion with nucleolytic enzymes. The DNAase I sensitivity of genes programmed to be expressed during myogenesis was tested in a cloned cell population of a myogenic cell line. It was found that these genes are not preferentially sensitive to DNAase I in the chromatin of proliferating mononucleated cells. They become DNAase I sensitive during terminal differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0020434043&partnerID=8YFLogxK
U2 - 10.1007/978-1-4899-5292-9_15
DO - 10.1007/978-1-4899-5292-9_15
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C2 - 6218737
AN - SCOPUS:0020434043
SN - 0065-2598
VL - 158
SP - 127
EP - 137
JO - Advances in Experimental Medicine and Biology
JF - Advances in Experimental Medicine and Biology
ER -