Analysis of myogenesis with recombinant DNA techniques.

D. Yaffe*, U. Nudel, H. Czosnek, R. Zakut, Y. Carmon, M. Shani

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Recombinant phages containing the rat skeletal muscle alpha-actin gene and the cytoplasmic beta-actin gene were isolated and the structure of these genes was determined. Both genes contain a large intron in the 5' untranslated region and smaller introns at codons 41, 267 and 327. In addition, the alpha-actin contains introns at codons 150 and 204 not present in the beta-actin gene, whereas the beta-actin gene contains an intron at codon 121. The evolutionary aspects of these findings are discussed. Active genes are organized in chromatin in a conformation which renders them preferentially sensitive to digestion with nucleolytic enzymes. The DNAase I sensitivity of genes programmed to be expressed during myogenesis was tested in a cloned cell population of a myogenic cell line. It was found that these genes are not preferentially sensitive to DNAase I in the chromatin of proliferating mononucleated cells. They become DNAase I sensitive during terminal differentiation.

Original languageEnglish
Pages (from-to)127-137
Number of pages11
JournalAdvances in Experimental Medicine and Biology
Volume158
DOIs
StatePublished - 1982

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