Ancymidol effects on oxidative stress and the regeneration potential of narcissus leaves in liquid culture

Jianxin Chen, Meira Ziv

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Scopus citations

Abstract

The addition of the growth retardant ancymidol (ANC) to Narcissus shoots cultured in liquid medium induced hyperhydric malformation. Morphogenetic changes were observed in the lower inner leaf sections isolated from hyperhydric shoots. The changes were manifested in meristematic centers formation on the basal proximal ends and later appeared all over the surface of the leaf sections. In non-hyperhydric leaf sections grown in liquid medium lacking ANC, only limited morphogenetic responses were observed. Superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) activities and hydrogen peroxide (H2O2) were examined in hyperhydric and non-hyperhydric isolated leaf sections. In ANC-treated hyperhydric leaf sections, SOD, APX, CAT activities and H2O2 level were lower than in control untreated normal leaf sections. The changes in antioxidant enzymatic activities were closely related to the onset of meristematic-center initiation and further bud development on Narcissus hyperhydric leaf sections. Buds were transplanted to agar medium and developed into normal shoots with bulblets.

Original languageEnglish
Title of host publicationIV International Symposium on In Vitro Culture and Horticultural Breeding
EditorsS. Sorvari, S. Karhu, S. Kanervo, S. Pihakaski
Pages299-302
Number of pages4
StatePublished - 2001

Publication series

NameActa Horticulturae
Volume560
ISSN (Print)0567-7572

Keywords

  • Ancymidol
  • Antioxidant enzymes
  • Hyperhydricity
  • Liquid culture
  • Narcissus

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