Ancymidol-enhanced hyperhydric malformation in relation to gibberellin and oxidative stress in liquid-cultured Narcissus leaves

Jianxin Chen*, Meira Ziv

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The growth retardant ancymidol inhibited gibberellin biosynthesis and enhanced hyperhydric malformation of Narcissus leaf sections cultured in liquid medium. Superoxide dismutase activities were examined by spectrophotometry and native polyacrylamide gel analysis, and gibberellin and hydrogen peroxide levels were determined spectrophotometrically in either hyperhydric or non-hyperhydric leaf sections. In ancymidol-treated hyperhydric leaf sections, superoxide dismutase activity and hydrogen peroxide levels were higher during the initial culture period, when hyperhydric malformation occurred, than in control untreated leaf sections. At a later stage, when the meristematic centers started to form on ancymidol-enhanced hyperhydric leaf sections, superoxide dismutase activity, hydrogen peroxide, and gibberellin levels were significantly lower in hyperhydric leaf sections than in non-treated leaf sections. The changes in superoxide dismutase activities, hydrogen peroxide, and gibberellin levels appeared to be related to hyperhydric malformation and meristematic center initiation.

Original languageEnglish
Pages (from-to)613-616
Number of pages4
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume40
Issue number6
DOIs
StatePublished - Nov 2004

Keywords

  • Ancymidol
  • Gibberellin
  • Hydrogen peroxide
  • Liquid culture
  • Narcissus tazetta
  • Superoxide dismutase

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