Anoxia followed by reoxygenation causes extensive damage to cellular components through generation of reactive oxygen intermediates. We examined cellular responses to oxidative stress after anoxia in cultured soybean or human fibroblast cells. Anoxia pretreatment protected soybean but not fibroblasts against H2O2 concentrations that induced programmed cell death in normoxic cells. H2O2 removal in anoxia-pretreated soybean cultures was faster. Protection was associated with increased action of alternative oxidase (AOX) and peroxidases. AOX inhibitors abolished the protective effect, while induction of AOX protected normoxic cells against H2O2. We propose that during anoxia, plant cells can prepare for reoxygenation injury by up-regulating their antioxidant capacity, and that AOX is involved in this process. Copyright (C) 2000 Federation of European Biochemical Societies.
Bibliographical noteFunding Information:
We are grateful to Dr. L. McIntosh (Michigan State University) for the AOX antibodies and to Dr. N. Kitrossky for HPLC analysis. This work was supported in part from the Israel Sciences Foundation (ISF) and from Binational Science Foundation (BSF).
- Alternative oxidase
- Hydrogen peroxide
- Oxidative stress
- Oxygen radical
- Reoxygenation injury