Anti-cancer binary system activated by bacteriophage HK022 integrase

Amer Elias; Natasha Gritsenko; Rena Gorovits; Itay Spector; Gali Prag; Ezra Yagil; Mikhail Kolot

doi:10.18632/oncotarget.25512

Anti-cancer binary system activated by bacteriophage HK022 integrase

Amer Elias, Natasha Gritsenko, Rena Gorovits, Itay Spector, Gali Prag, Ezra Yagil, Mikhail Kolot^*

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The binary system presented in this work is based on the bacteriophage HK022 integrase recombinase that activates the expression of a silenced Diphtheria toxin gene, both controlled by the cancer specific hTERT promoter. Using a lung cancer mice model, assays of different apoptotic and anti-apoptotic factors have demonstrated that the Integrase based binary system is highly specific towards cancer cells and more efficient compared to the conventional mono system whose toxin is directly expressed under hTERT. In a mice survival test, this binary system demonstrated longer persistence compared to the untreated and the mono treated ones. The reason underlying the advantage of this binary system over the mono system seems to be an overexpression of various hTERT suppressing factors induced by the mono system.

Original language	English
Pages (from-to)	27487-27501
Number of pages	15
Journal	Oncotarget
Volume	9
Issue number	44
DOIs	https://doi.org/10.18632/oncotarget.25512
State	Published - 1 Jun 2018

Bibliographical note

Publisher Copyright:
© Elias et al.

Keywords

Cancer therapy
Coliphage HK022 integrase
DTA toxin
Lung cancer
Site-specific recombination

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.18632/oncotarget.25512

Cite this

@article{79f796aaf71c448cab487995b0c42e9a,

title = "Anti-cancer binary system activated by bacteriophage HK022 integrase",

abstract = "The binary system presented in this work is based on the bacteriophage HK022 integrase recombinase that activates the expression of a silenced Diphtheria toxin gene, both controlled by the cancer specific hTERT promoter. Using a lung cancer mice model, assays of different apoptotic and anti-apoptotic factors have demonstrated that the Integrase based binary system is highly specific towards cancer cells and more efficient compared to the conventional mono system whose toxin is directly expressed under hTERT. In a mice survival test, this binary system demonstrated longer persistence compared to the untreated and the mono treated ones. The reason underlying the advantage of this binary system over the mono system seems to be an overexpression of various hTERT suppressing factors induced by the mono system.",

keywords = "Cancer therapy, Coliphage HK022 integrase, DTA toxin, Lung cancer, Site-specific recombination",

author = "Amer Elias and Natasha Gritsenko and Rena Gorovits and Itay Spector and Gali Prag and Ezra Yagil and Mikhail Kolot",

note = "Publisher Copyright: {\textcopyright} Elias et al.",

year = "2018",

month = jun,

day = "1",

doi = "10.18632/oncotarget.25512",

language = "אנגלית",

volume = "9",

pages = "27487--27501",

journal = "Oncotarget",

issn = "1949-2553",

publisher = "Impact Journals LLC",

number = "44",

}

TY - JOUR

T1 - Anti-cancer binary system activated by bacteriophage HK022 integrase

AU - Elias, Amer

AU - Gritsenko, Natasha

AU - Gorovits, Rena

AU - Spector, Itay

AU - Prag, Gali

AU - Yagil, Ezra

AU - Kolot, Mikhail

N1 - Publisher Copyright: © Elias et al.

PY - 2018/6/1

Y1 - 2018/6/1

N2 - The binary system presented in this work is based on the bacteriophage HK022 integrase recombinase that activates the expression of a silenced Diphtheria toxin gene, both controlled by the cancer specific hTERT promoter. Using a lung cancer mice model, assays of different apoptotic and anti-apoptotic factors have demonstrated that the Integrase based binary system is highly specific towards cancer cells and more efficient compared to the conventional mono system whose toxin is directly expressed under hTERT. In a mice survival test, this binary system demonstrated longer persistence compared to the untreated and the mono treated ones. The reason underlying the advantage of this binary system over the mono system seems to be an overexpression of various hTERT suppressing factors induced by the mono system.

AB - The binary system presented in this work is based on the bacteriophage HK022 integrase recombinase that activates the expression of a silenced Diphtheria toxin gene, both controlled by the cancer specific hTERT promoter. Using a lung cancer mice model, assays of different apoptotic and anti-apoptotic factors have demonstrated that the Integrase based binary system is highly specific towards cancer cells and more efficient compared to the conventional mono system whose toxin is directly expressed under hTERT. In a mice survival test, this binary system demonstrated longer persistence compared to the untreated and the mono treated ones. The reason underlying the advantage of this binary system over the mono system seems to be an overexpression of various hTERT suppressing factors induced by the mono system.

KW - Cancer therapy

KW - Coliphage HK022 integrase

KW - DTA toxin

KW - Lung cancer

KW - Site-specific recombination

UR - http://www.scopus.com/inward/record.url?scp=85048246555&partnerID=8YFLogxK

U2 - 10.18632/oncotarget.25512

DO - 10.18632/oncotarget.25512

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:85048246555

SN - 1949-2553

VL - 9

SP - 27487

EP - 27501

JO - Oncotarget

JF - Oncotarget

IS - 44

ER -

Anti-cancer binary system activated by bacteriophage HK022 integrase

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this