Abstract
Heterologous anti-idiotypic reagents have been prepared against a BALB/c anti-swine MHC (SLA(d)) monoclonal antibody (74-11-10) in order to test for possible interspecies idiotypic cross-reactions of anti-MHC antibodies. Using these reagents to examine xenoantisera produced in BALB/c mice immunized with swine SLA(dd) peripheral blood lymphocytes, all animals tested were found to express detectable levels of the 74-11-10 idiotype (Id). The Id could also be detected in one out of five BALB/c mice immunized with swine SLA(cc) PBL. Swine anti-SLA(dd) alloantibodies were also tested, but failed to show detectable levels of the 74-11-10 Id. The in vivo administration of anti-idiotypic reagents to BALB/c mice induced detectable levels of 74-11-10 Id positive antibodies that bound specifically to SLA(dd) PBL. Similar treatment of SLA(gg) swine (recombinant swine expressing the class I MHC molecules of c) with anti-idiotypic antibodies failed to induce anti-SLA(d) antibody activity. These results thus indicate that 74-11-10 represents a shared idiotype of BALB/c anti-SLA(dd) antibodies. The presence of 74-11-10 Id in one mouse immunized with SLA(cc) PBL suggests that the failure of pigs to express the 74-11-10 Id following treatment with anti-idiotypic antibodies may be the result of tolerance rather than absence of the relevant variable region gene(s).
| Original language | English |
|---|---|
| Pages (from-to) | 607-613 |
| Number of pages | 7 |
| Journal | Immunology |
| Volume | 58 |
| Issue number | 4 |
| State | Published - 1986 |
| Externally published | Yes |