TY - JOUR
T1 - Anti-T lymphocyte globulin (ATG) induces generation of regulatory T cells, at least part of them express activated CD44
AU - Shimony, Orly
AU - Nagler, Arnon
AU - Gellman, Yechiel N.
AU - Refaeli, Efrat
AU - Rosenblum, Nir
AU - Eshkar-Sebban, Lora
AU - Yerushalmi, Ronit
AU - Shimoni, Avichai
AU - Lytton, Simon D.
AU - Stanevsky, Anfisa
AU - Or, Reuven
AU - Naor, David
PY - 2012/2
Y1 - 2012/2
N2 - We show here that the anti-T lymphocyte immunoglobulin (ATG) can induce Treg cells following 24-h incubation in human peripheral blood mononuclear cells (PBMCs). The ATG-induced Treg cells express known cell surface markers (e.g., CD25, FoxP3) and suppress the proliferation of autologous responder PBMCs, stimulated with allogeneic PBMCs, when added into the mixed lymphocyte culture (MLC) at zero time point or 48 h later. We expanded the characteristics of the ATG-induced human Treg cells by showing that they express a novel biomarker designated "activated CD44". ATG-induced Treg cells retain their suppressor function after freezing and thawing or irradiation. Suppression of MLC by ATG-induced Treg cells is consistently seen when the Treg cells and the responder cells were derived from the same donor, but not when they derived from different donors. Finally, patients undergoing stem cell transplantation and conditioned with ATG generate in vivo Treg cells that suppress MLC.
AB - We show here that the anti-T lymphocyte immunoglobulin (ATG) can induce Treg cells following 24-h incubation in human peripheral blood mononuclear cells (PBMCs). The ATG-induced Treg cells express known cell surface markers (e.g., CD25, FoxP3) and suppress the proliferation of autologous responder PBMCs, stimulated with allogeneic PBMCs, when added into the mixed lymphocyte culture (MLC) at zero time point or 48 h later. We expanded the characteristics of the ATG-induced human Treg cells by showing that they express a novel biomarker designated "activated CD44". ATG-induced Treg cells retain their suppressor function after freezing and thawing or irradiation. Suppression of MLC by ATG-induced Treg cells is consistently seen when the Treg cells and the responder cells were derived from the same donor, but not when they derived from different donors. Finally, patients undergoing stem cell transplantation and conditioned with ATG generate in vivo Treg cells that suppress MLC.
KW - Activated CD44
KW - FoxP3
KW - Hematological diseases
KW - Regulatory T cells
UR - http://www.scopus.com/inward/record.url?scp=84862259635&partnerID=8YFLogxK
U2 - 10.1007/s10875-011-9599-2
DO - 10.1007/s10875-011-9599-2
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C2 - 21979414
AN - SCOPUS:84862259635
SN - 0271-9142
VL - 32
SP - 173
EP - 188
JO - Journal of Clinical Immunology
JF - Journal of Clinical Immunology
IS - 1
ER -