Anti-viral RNAi nanoparticles protect shrimp against white spot disease

Shai Ufaz, Adi Balter, Chen Tzror, Shai Einbender, Ori Koshet, Janna Shainsky-Roitman, Zvi Yaari, Avi Schroeder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Nearly 20% of cultured shrimp die every year due to viral diseases. In this study, we evaluated the capacity of nanoparticulate RNA interference (RNAi) to down-regulate genes in Penaeus vannamei shrimp and protect shrimp against white spot syndrome virus (WSSV, i.e. white spot disease). Using a reporter target gene, Rab7, we show that the length of the administered dsRNA correlates with gene knockdown. We found that 250 bp-long dsRNA strands knocked down gene expression most effectively, followed by 125 and 70 bp-long strands. The 21 bp long strands did not downregulate the gene. We also show gene downregulation to be concentration dependent. Even at low RNA concentrations of 0.01 μg per gram-body-weight gene knockdown exceeded 80%. Knockdown levels were similar in multiple organs, including the gills, gut, hepato-pancreas, pleopods and muscle. Gene knockdown lasted for one month, after which gene expression recuperated. To protect the RNA molecules from enzymatic degradation, we complexed the RNA with the cationic polysaccharide chitosan, forming 90-200 nm particles that facilitated efficient RNAi in vivo. In a double-blinded viral challenge test, RNAi targeting viral-protein 28 (VP28) protected the shrimp against WSSV infection. Survival of animals treated with RNAi nanoparticles exceeded 95% compared to no survival in the untreated controls. Nanoparticulate RNAi is an effective modality for protecting against viral diseases.

Original languageAmerican English
Pages (from-to)38-48
Number of pages11
JournalMolecular Systems Design and Engineering
Volume3
Issue number1
DOIs
StatePublished - Feb 2018
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2018 The Royal Society of Chemistry.

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